Lowe A M, Beattie D T, Deresiewicz R L
Infectious Disease Division, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Mol Microbiol. 1998 Mar;27(5):967-76. doi: 10.1046/j.1365-2958.1998.00741.x.
We have applied in vivo expression technology (IVET) to the study of staphylococcal virulence. Using a promoter trap that relies on genetic recombination as a reporter of gene expression, we identified 45 staphylococcal genes that are induced during infection in a murine renal abscess model. Of these, only six were known previously; 11 others have homology to known non-staphylococcal genes. The known staphylococcal genes include agrA, part of a key locus regulating numerous virulence products, and a glycerol ester hydrolase, which may enhance staphylococcal survival in abscesses. We constructed 11 strains containing mutations in previously unknown ivi genes. Of these strains, seven were significantly attenuated in virulence compared with the wild-type parent. The mutagenized ivi genes may encode novel staphylococcal virulence factors.
我们已将体内表达技术（IVET）应用于葡萄球菌毒力的研究。利用一种依赖基因重组作为基因表达报告子的启动子捕获技术，我们在小鼠肾脓肿模型感染过程中鉴定出45个被诱导表达的葡萄球菌基因。其中，只有6个基因先前已知；另外11个基因与已知的非葡萄球菌基因具有同源性。已知的葡萄球菌基因包括agrA，它是调控众多毒力产物的关键位点的一部分，以及一种甘油酯水解酶，其可能增强葡萄球菌在脓肿中的存活能力。我们构建了11株在先前未知的ivi基因中含有突变的菌株。与野生型亲本相比，这些菌株中有7株的毒力显著减弱。诱变的ivi基因可能编码新型葡萄球菌毒力因子。
