Bagetta G, Corasaniti M T, Paoletti A M, Berliocchi L, Nisticò R, Giammarioli A M, Malorni W, Finazzi-Agrò A
Department of Pharmaco-Biology, University of Calabria at Cosenza, Italy.
Biochem Biophys Res Commun. 1998 Mar 27;244(3):819-24. doi: 10.1006/bbrc.1998.8321.
The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclooxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison to control, bovine serum albumin (100 ng, given i.c.v. for up to 7 days) treated animals (n = 6), a single daily injection of the viral protein for 7 consecutive days enhanced the number of COX-2 immunoreactive cells in the brain cortex of rats (n = 6 per group) and this was accompanied by a 50% increase over control PGE2 content in whole brain tissue homogenates (n = 6). In another series of experiments, pretreatment of rats (n = 6) with indomethacin (6.0 mg/kg given i.p. 1 h before gp120 injection), an inhibitor COX activity, prevented apoptotic death typically produced by gp120 in the neocortex of rat suggesting that enhancement of COX-2 expression may be involved in the mechanisms of apoptosis yielded by the HIV-1 coat protein.
通过免疫组织化学方法，研究了成年大鼠脑内连续7天每天脑室注射100 ng人免疫缺陷病毒1型（HIV-1）重组蛋白gp120对环氧合酶-2（COX-2）表达的影响。与对照组[脑室内注射牛血清白蛋白（100 ng，连续7天）处理的动物（n = 6）]相比，连续7天每天单次注射病毒蛋白可增加大鼠脑皮质中COX-2免疫反应性细胞的数量（每组n = 6），并且全脑组织匀浆中的PGE2含量比对照组增加了50%（n = 6）。在另一系列实验中，用吲哚美辛（在注射gp120前1小时腹腔注射6.0 mg/kg）对大鼠（n = 6）进行预处理，吲哚美辛是一种COX活性抑制剂，可防止gp120通常在大鼠新皮质中产生的凋亡性死亡，这表明COX-2表达的增强可能参与了HIV-1外壳蛋白诱导的凋亡机制。
