Pyapali G K, Turner D A, Williams C L, Meck W H, Swartzwelder H S
Department of Neurosurgery, Duke University, Durham 27705, USA.
J Neurophysiol. 1998 Apr;79(4):1790-6. doi: 10.1152/jn.1998.79.4.1790.
Choline supplementation during gestation in rats leads to augmentation of spatial memory in adulthood. We hypothesized that prenatal (E12-E17) choline supplementation in the rat would lead to an enhancement of hippocampal synaptic plasticity as assessed by long-term potentiation (LTP) at 3-4 mo of age. LTP was assessed blindly in area CA1 of hippocampal slices with first suprathreshold (above threshold for LTP generation in control slices) theta-burst stimulus trains. The magnitude of potentiation after these stimuli was not different between slices from control and prenatally choline supplemented animals. Next, threshold (reliably leading to LTP generation in control slices) or subthreshold theta-burst stimulus trains were applied to slices from control, prenatally choline-supplemented, and prenatally choline-deprived rats. Threshold level stimulus trains induced LTP in slices from both the control and choline-supplemented rats but not in those from the choline-deficient rats. Subthreshold stimulus trains led to LTP induction in slices from prenatally choline-supplemented rats only. These observations indicate that prenatal dietary manipulation of the amino acid, choline, leads to subsequent significant alterations of LTP induction threshold in adult animals.
孕期给大鼠补充胆碱会增强其成年后的空间记忆。我们假设，孕期（胚胎期第12 - 17天）给大鼠补充胆碱会增强海马体突触可塑性，这通过3 - 4月龄时的长时程增强（LTP）来评估。在海马体切片的CA1区域，采用首次阈上（高于对照切片中LTP产生阈值）的theta波爆发刺激序列对LTP进行盲法评估。在对照动物和孕期补充胆碱的动物的切片之间，这些刺激后的增强幅度没有差异。接下来，将阈值（在对照切片中可靠地导致LTP产生）或阈下theta波爆发刺激序列应用于对照、孕期补充胆碱和孕期胆碱缺乏大鼠的切片。阈值水平的刺激序列在对照大鼠和补充胆碱大鼠的切片中诱导出LTP，但在胆碱缺乏大鼠的切片中未诱导出LTP。阈下刺激序列仅在孕期补充胆碱大鼠的切片中导致LTP诱导。这些观察结果表明，孕期对氨基酸胆碱进行饮食调控会导致成年动物后续LTP诱导阈值发生显著改变。
