Ryan M, Murphy G, Ryan E, Nilsson L, Shackley F, Gothefors L, Oymar K, Miller E, Storsaeter J, Mills K H
Department of Biology, National University of Ireland, Maynooth, Ireland.
Immunology. 1998 Jan;93(1):1-10. doi: 10.1046/j.1365-2567.1998.00401.x.
Recent clinical trials have demonstrated that new generation acellular pertussis vaccines can confer protection against whooping cough. However, the mechanism of protective immunity against Bordetella pertussis infection induced by vaccination remains to be defined. We have examined cellular immune responses in children immunized with a range of acellular and whole cell pertussis vaccines. Immunization of children with a potent whole-cell vaccine induced B. pertussis-specific T cells that secreted interferon-gamma (IFN-gamma), but not interleukin-5 (IL-5). In contrast, T cells from children immunized with acellular pertussis vaccines secreted IFN-gamma and/or IL-5 following stimulation with B. pertussis antigens in vitro. These observations suggest that protective immunity conferred by whole-cell vaccines, like natural immunity, is mediated by type 1 T cells, whereas the mechanism of immune protection generated with acellular vaccines may be more heterogeneous, involving T cells that secreted type 1 and type 2 cytokines.
近期的临床试验表明，新一代无细胞百日咳疫苗可提供针对百日咳的保护。然而，接种疫苗诱导的针对百日咳博德特氏菌感染的保护性免疫机制仍有待确定。我们检测了接种一系列无细胞和全细胞百日咳疫苗的儿童的细胞免疫反应。用强效全细胞疫苗免疫儿童可诱导出分泌γ干扰素（IFN-γ）但不分泌白细胞介素-5（IL-5）的百日咳博德特氏菌特异性T细胞。相比之下，接种无细胞百日咳疫苗的儿童的T细胞在体外用百日咳博德特氏菌抗原刺激后会分泌IFN-γ和/或IL-5。这些观察结果表明，全细胞疫苗赋予的保护性免疫，如同天然免疫一样，由1型T细胞介导，而无细胞疫苗产生的免疫保护机制可能更为多样，涉及分泌1型和2型细胞因子的T细胞。
