Yang H, Parkhouse R M
Immunology Division, BBSRC Institute for Animal Health, Pirbright, UK.
Immunology. 1998 Jan;93(1):26-32. doi: 10.1046/j.1365-2567.1998.00396.x.
A panel of monoclonal antibodies directed to different epitopes of porcine CD3 were employed to investigate stimulation requirements of porcine T lymphocytes. It was found that epitope specificity was an important property of the anti-CD3 antibodies that determined the requirements for T-cell proliferation. Thus, T-cell proliferation induced by triggering different CD3 epitopes showed three different requirements: (a) proliferation induced by the most insensitive epitope required both epitope ligation and some unknown additional signal(s); (b) proliferation induced by the most common epitopes only required epitope ligation, either by monocytes or by immobilization; (c) proliferation induced by the most sensitive epitope required neither epitope ligation nor participation of antigen-presenting cells (APC). These findings may help to explain the previous confusion over the requirements for T-cell activation through the CD3 pathway. Finally, the above conclusions apply only to alpha beta T cells, as porcine gamma delta T cells, either in bulk culture or isolated, did not proliferate in response to anti-CD3 stimulation. Therefore, the mechanism underlying gamma delta T-cell activation may be different from that of alpha beta T cells.
使用一组针对猪CD3不同表位的单克隆抗体来研究猪T淋巴细胞的刺激需求。发现表位特异性是抗CD3抗体的一个重要特性，它决定了T细胞增殖的需求。因此，触发不同CD3表位诱导的T细胞增殖表现出三种不同的需求：(a) 由最不敏感表位诱导的增殖既需要表位连接，也需要一些未知的额外信号；(b) 由最常见表位诱导的增殖仅需要表位连接，可通过单核细胞或固定化实现；(c) 由最敏感表位诱导的增殖既不需要表位连接，也不需要抗原呈递细胞(APC)的参与。这些发现可能有助于解释先前关于通过CD3途径激活T细胞的需求的困惑。最后，上述结论仅适用于αβ T细胞，因为无论是在批量培养还是分离状态下，猪γδ T细胞均不响应抗CD3刺激而增殖。因此，γδ T细胞激活的潜在机制可能与αβ T细胞不同。
