Kasahara Y, Chen C H, Cooper M D
Department of Medicine, University of Alabama at Birmingham 35294-3300.
Eur J Immunol. 1993 Sep;23(9):2230-6. doi: 10.1002/eji.1830230927.
These studies analyze growth requirements for the normal gamma delta T cell population in peripheral lymphoid tissues. Avian gamma delta T cells can respond well to T cell mitogens in the presence of alpha beta T cells, but our studies indicate that they do not grow well alone. Exogenous growth factors were required in order for gamma delta T cells to proliferate in response to receptor ligation by anti-T cell receptor antibodies or other T cell mitogens. Interleukin-2 was implicated as one of the necessary growth factors that the gamma delta cells cannot produce adequately on their own. The response to dual stimulation (receptor ligation plus exogenous T cell factors) was attributable to a discrete subpopulation of gamma delta T cells that could be identified by their cell surface CD8, major histocompatibility complex class II expression and relative increase in cell size. Conversely, non-responsive gamma delta T cells did not exhibit these activation markers. These observations suggest a physiological basis for the relatively late appearance of gamma delta T cells in inflammatory responses and their failure as a population to match the growth potential of alpha beta T cells. More importantly, the results imply that the biological role of gamma delta T cells must be understood within the context of their interaction with alpha beta T cells.
这些研究分析了外周淋巴组织中正常γδ T细胞群体的生长需求。在存在αβ T细胞的情况下，禽类γδ T细胞能够很好地对T细胞有丝分裂原作出反应，但我们的研究表明，它们单独生长时情况不佳。为了使γδ T细胞在抗T细胞受体抗体或其他T细胞有丝分裂原介导的受体连接反应中增殖，需要外源性生长因子。白细胞介素-2被认为是γδ细胞自身无法充分产生的必要生长因子之一。对双重刺激（受体连接加外源性T细胞因子）的反应归因于γδ T细胞的一个离散亚群，可通过其细胞表面CD8、主要组织相容性复合体II类表达以及细胞大小的相对增加来识别。相反，无反应的γδ T细胞不表现出这些激活标志物。这些观察结果为γδ T细胞在炎症反应中相对较晚出现以及作为一个群体无法与αβ T细胞的生长潜力相匹配提供了生理基础。更重要的是，结果表明γδ T细胞的生物学作用必须在其与αβ T细胞相互作用的背景下加以理解。
