Sharma A, Gaikwad S, Rajput S
Department of Post-Graduate Studies and Research in Biological Sciences, R.D. University, Jabalpur, India.
Indian J Exp Biol. 1998 Jan;36(1):76-85.
The phosphomonoesterases catalyse the hydrolysis of primary esters of phosphoric acid which help the bacteria to survive in phosphate stressed environment. Ninety-five bacterial isolates were obtained from domestic sewage and industrial effluents of gelatine and soap factories at Jabalpur on a medium enriched with phosphate and were screened for phosphatase production. The phosphatase producers were tentatively identified as Escherichia coli, Vibrio vulnificus, Aeromonas hydrophila, Staphylococcus aureus, Pseudomonas maltophilia and Micrococcus varians. The in vitro studies on the production of phosphomonoesterases by bacteria was conducted. The maximum alkaline phosphatase production was recorded on 8th day of incubation by E.coli and P.maltophilia, on 10th day of incubation by V.vulnificus while M.varians and P.maltophilia produced higher acid phosphatase on 4th and 10th day of incubation respectively. The detailed investigations were done to find out the effect of various physical and chemical factors on phosphomonoesterases activity and the optimum conditions required for enzyme activity.
磷酸单酯酶催化磷酸一酯的水解，这有助于细菌在磷酸盐胁迫环境中生存。从贾巴尔普尔的明胶和肥皂厂的生活污水和工业废水中，在富含磷酸盐的培养基上获得了95株细菌分离株，并筛选了磷酸酶的产生情况。磷酸酶产生菌初步鉴定为大肠杆菌、创伤弧菌、嗜水气单胞菌、金黄色葡萄球菌、嗜麦芽窄食单胞菌和变异微球菌。进行了关于细菌产生磷酸单酯酶的体外研究。大肠杆菌和嗜麦芽窄食单胞菌在培养第8天碱性磷酸酶产量最高，创伤弧菌在培养第10天产量最高，而变异微球菌和嗜麦芽窄食单胞菌分别在培养第4天和第10天产生较高的酸性磷酸酶。进行了详细研究以找出各种物理和化学因素对磷酸单酯酶活性的影响以及酶活性所需的最佳条件。
