Satta G, D'Andrea L, Grazi G, Soro O, Varaldo P E
Institute of Microbiology, Faculty of Medicine, Catholic University of the Sacred Heart, Rome, Italy.
Int J Syst Bacteriol. 1993 Oct;43(4):813-8. doi: 10.1099/00207713-43-4-813.
The phosphatase activities of 114 micrococcal strains belonging to seven different species and of an additional 150 unspeciated micrococcal strains were evaluated on solid media at various pHs containing or not containing phosphates. In the presence of phosphates, only nine strains (five unspeciated strains, one Micrococcus luteus strain, and three Micrococcus varians strains) yielded a positive reaction on plates at pH 8. In media (at pH 8) deprived of phosphates, in contrast, all but 15 strains demonstrated clear-cut phosphatase activity. Acid phosphatase could not be evaluated on solid media since none of the strains grew satisfactorily on plates at pH 5. The phosphatase activities of seven (one or two for each species, which included phosphatase-negative strains) of the strains whose colonies proved phosphatase negative at pH 8 and of 18 (two or three strains per species) of those with phosphatase-positive colonies were evaluated at pH 5 and 8.5 in toluene-treated cells which had been grown in liquid media at pH 7 containing or not containing phosphates. All strains demonstrated distinct phosphatase activity at both pHs when grown in media not containing phosphates. In contrast, when strains were grown in the presence of such substances, virtually no activity was observed at pH 8.5, and, generally, a much reduced activity was observed at pH 5. The phosphatase activity of micrococci of the various species (three to eight strains per species) was also compared with that of staphylococci of different species (5 to 10 strains per species) by the methyl green-phenolphthalein diphosphate method, the sensitivity of which can be varied by using different enzyme substrates.(ABSTRACT TRUNCATED AT 250 WORDS)
对属于7个不同种的114株微球菌菌株以及另外150株未分类的微球菌菌株，在含有或不含磷酸盐的不同pH值的固体培养基上评估其磷酸酶活性。在有磷酸盐存在的情况下，只有9株菌株（5株未分类菌株、1株藤黄微球菌菌株和3株变异微球菌菌株）在pH 8的平板上产生阳性反应。相比之下，在不含磷酸盐的培养基（pH 8）中，除15株菌株外，所有菌株都表现出明显的磷酸酶活性。由于没有菌株在pH 5的平板上生长良好，因此无法在固体培养基上评估酸性磷酸酶。对7株（每个种1或2株，包括磷酸酶阴性菌株）在pH 8时菌落证明为磷酸酶阴性的菌株以及18株（每个种2或3株）菌落为磷酸酶阳性的菌株，在pH 5和8.5条件下，对在含有或不含磷酸盐的pH 7液体培养基中生长的经甲苯处理的细胞进行磷酸酶活性评估。当在不含磷酸盐的培养基中生长时，所有菌株在这两个pH值下均表现出明显的磷酸酶活性。相反，当菌株在这些物质存在的情况下生长时，在pH 8.5时几乎没有观察到活性，并且通常在pH 5时观察到活性大大降低。还通过甲基绿 - 酚酞二磷酸法比较了不同种微球菌（每个种3至8株）和不同种葡萄球菌（每个种5至10株）的磷酸酶活性，该方法的灵敏度可通过使用不同的酶底物来改变。（摘要截短于250字）
