Vray B, Hoebeke J, Saint-Guillain M, Leloup R, Strosberg A D
Laboratory of Microbiology and Immunology, Faculty of Medicine, Free University of Brussels.
Scand J Immunol. 1980;11(2):147-53. doi: 10.1111/j.1365-3083.1980.tb00220.x.
We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate-buffered saline. After washing of the phagocytes, the fluorescence of those that have ingested the labelled bacteria is measured with a spectrofluorimeter. We report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index.
我们描述了一种用于细菌吞噬作用的新型定量荧光测定法。将荧光素标记的细菌（溶壁微球菌）悬浮液与吞噬细胞混合并孵育。用多聚甲醛固定后，用溶菌酶在磷酸盐缓冲盐水中的溶液裂解过量未被吞噬和吸附的细菌。洗涤吞噬细胞后，用荧光分光光度计测量摄取了标记细菌的细胞的荧光。我们报告了用不同类型吞噬细胞获得的结果，这些结果表明该方法可用于敏感性、饱和度和动力学研究以及吞噬指数的计算。
