Smet E G, De Smet W, Brys L, De Baetselier P C
Immunology. 1986 Nov;59(3):419-25.
A mouse monoclonal (Mab.198, IgG1) was generated that recognizes an epitope expressed on rabbit peripheral phagocytes and tissue macrophages. The membrane antigen recognized by Mab.198 consisted of two polypeptide chains of 165,000 and 95,000 molecular weight (MW). This Mab efficiently inhibited complement receptor-mediated granulocyte functions such as phagocytosis of complement-opsonized particulate antigens and zymosan-induced chemiluminescent responses. Fc receptor-mediated phagocyte functions were, on the contrary, barely affected by Mab.198. The cellular distribution, molecular structure and functional characteristics of the membrane antigen defined by Mab.198 suggested that this antibody recognizes the complement type 3 receptor (CR3). We found that Mab.M1/70, specific for mouse CR3 (i.e. CD 11 or Mac-1 antigen), also binds on rabbit phagocytes. However, this Mab recognizes a different CR3 epitope than Mab.198 as shown by cross-blocking experiments. This anti-rabbit CR3 Mab will be useful in the characterization of rabbit complement receptors and their involvement in immune reactions.
产生了一种小鼠单克隆抗体（Mab.198，IgG1），它识别兔外周吞噬细胞和组织巨噬细胞上表达的一个表位。Mab.198识别的膜抗原由两条分子量分别为165,000和95,000的多肽链组成。该单克隆抗体有效抑制补体受体介导的粒细胞功能，如补体调理的颗粒抗原的吞噬作用和酵母聚糖诱导的化学发光反应。相反，Fc受体介导的吞噬细胞功能几乎不受Mab.198的影响。由Mab.198定义的膜抗原的细胞分布、分子结构和功能特性表明，该抗体识别补体3型受体（CR3）。我们发现，对小鼠CR3（即CD 11或Mac-1抗原）具有特异性的Mab.M1/70也能与兔吞噬细胞结合。然而，如交叉阻断实验所示，该单克隆抗体识别的CR3表位与Mab.198不同。这种抗兔CR3单克隆抗体将有助于表征兔补体受体及其在免疫反应中的作用。
