一种古菌趋氧性转导蛋白结合了真核细胞色素c氧化酶的亚基I核心结构和细菌甲基接受趋化蛋白。
An archaeal aerotaxis transducer combines subunit I core structures of eukaryotic cytochrome c oxidase and eubacterial methyl-accepting chemotaxis proteins.
作者信息
Brooun A, Bell J, Freitas T, Larsen R W, Alam M
机构信息
Department of Microbiology, University of Hawaii, Honolulu 96822, USA.
出版信息
J Bacteriol. 1998 Apr;180(7):1642-6. doi: 10.1128/JB.180.7.1642-1646.1998.
Abstract
Signal transduction in the archaeon Halobacterium salinarum is mediated by three distinct subfamilies of transducer proteins. Here we report the complete htrVIII gene sequence and present analysis of the encoded primary structure and its functional features. HtrVIII is a 642-amino-acid protein and belongs to halobacterial transducer subfamily B. At the N terminus, the protein contains six transmembrane segments that exhibit homology to the heme-binding sites of the eukaryotic cytochrome c oxidase. The C-terminal domain has high homology with the eubacterial methyl-accepting chemotaxis protein. The HtrVIII protein mediates aerotaxis: a strain with a deletion of the htrVIII gene loses aerotaxis, while an overproducing strain exhibits stronger aerotaxis. We also demonstrate that HtrVIII is a methyl-accepting protein and demethylates during the aerotaxis response.
摘要
嗜盐古菌盐生盐杆菌中的信号转导由三种不同的转导蛋白亚家族介导。在此，我们报告完整的htrVIII基因序列，并对编码的一级结构及其功能特征进行分析。HtrVIII是一种含有642个氨基酸的蛋白质，属于盐杆菌转导蛋白亚家族B。在N端，该蛋白质包含六个跨膜片段，与真核细胞色素c氧化酶的血红素结合位点具有同源性。C端结构域与真细菌甲基接受趋化蛋白具有高度同源性。HtrVIII蛋白介导趋氧性：htrVIII基因缺失的菌株失去趋氧性，而过表达菌株表现出更强的趋氧性。我们还证明HtrVIII是一种甲基接受蛋白，在趋氧性反应过程中会发生去甲基化。
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本文引用的文献
1
The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior.Aer蛋白和丝氨酸化学感受器Tsr能独立感知细胞内的能量水平，并为大肠杆菌的行为转导氧气、氧化还原和能量信号。
Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10541-6. doi: 10.1073/pnas.94.20.10541.
2
A signal transducer for aerotaxis in Escherichia coli.大肠杆菌中趋氧性的信号转导器。
J Bacteriol. 1997 Jun;179(12):4075-9. doi: 10.1128/jb.179.12.4075-4079.1997.
3
Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.嗜盐古菌盐生盐杆菌中可溶性转导蛋白HtrXI的一级结构与功能分析
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4
Seventy-five percent accuracy in protein secondary structure prediction.蛋白质二级结构预测的准确率达75%。
Proteins. 1997 Mar;27(3):329-35. doi: 10.1002/(sici)1097-0134(199703)27:3<329::aid-prot1>3.0.co;2-8.
5
Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence.将非局部相互作用纳入从氨基酸序列预测蛋白质二级结构的过程中。
Protein Eng. 1996 Feb;9(2):133-42. doi: 10.1093/protein/9.2.133.
6
SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments.SOPMA：通过多序列比对的一致性预测显著改善蛋白质二级结构预测。
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7
A family of halobacterial transducer proteins.嗜盐菌转导蛋白家族。
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8
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9
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10
Prediction of protein secondary structure at better than 70% accuracy.蛋白质二级结构预测准确率高于70%。
J Mol Biol. 1993 Jul 20;232(2):584-99. doi: 10.1006/jmbi.1993.1413.
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