Scotland S M, Rowe B, Smith H R, Willshaw G A, Gross R J
Division of Enteric Pathogens, Central Public Health Laboratory, London.
J Med Microbiol. 1988 Apr;25(4):237-43. doi: 10.1099/00222615-25-4-237.
Faecal specimens from 66 children with haemolytic uraemic syndrome in the United Kingdom were examined for strains of Escherichia coli producing Vero cytotoxin (VT). Initially, conventional bacteriological methods were used to identify colonies of E. coli which were then tested for VT production. Subsequently, specific DNA probes for VT1 and VT2 were used in hybridisation tests to detect VT-producing E. coli (VTEC). VTEC strains were isolated from 19 cases and in 15 they belonged to serogroup O157. Fourteen of these O157 strains possessed the flagellar antigen H7 and one was non-motile. The VTEC strains from the remaining four cases belonged to serotypes O26:H11, O104:H2, O153:H25, and O163:H19 together with a rough VT+ strain with flagellar antigen H51. The O157 strains hybridised with either the VT2 probe or both VT1 and VT2 probes. The other VTEC strains hybridised with either the VT1 or VT2 probe. Confirmation of the production of VT1 and VT2 in vivo was obtained by the neutralisation of faecal VT with specific antisera raised against these two cytotoxins.
对来自英国66名溶血尿毒综合征患儿的粪便标本进行了检测，以查找产生维罗细胞毒素（VT）的大肠杆菌菌株。最初，使用传统细菌学方法鉴定大肠杆菌菌落，然后检测其是否产生VT。随后，使用针对VT1和VT2的特异性DNA探针进行杂交试验，以检测产VT大肠杆菌（VTEC）。从19例病例中分离出VTEC菌株，其中15例属于O157血清群。这些O157菌株中有14株具有鞭毛抗原H7，1株无运动性。其余4例的VTEC菌株分别属于血清型O26:H11、O104:H2、O153:H25和O163:H19，以及1株带有鞭毛抗原H51的粗糙型VT+菌株。O157菌株与VT2探针或VT1和VT2探针均杂交。其他VTEC菌株与VT1或VT2探针杂交。通过用针对这两种细胞毒素产生的特异性抗血清中和粪便中的VT，证实了体内VT1和VT2的产生。
