Watanabe Y, Konishi M, Shimada M, Tsuji H, Nishio H, Suzuki K, Iwamoto S
Department of Anatomy, Osaka Medical College, Japan.
Kaibogaku Zasshi. 1998 Feb;73(1):33-41.
This study was designed to estimate the age at the death of cadavers by histomorphometry of the femur. Samples from 72 Japanese males from 43 days to 92 years old and 26 females from 2 to 88 years old were used. The thickness of sections was adjusted to 50-70 microns by grinding with sandpaper. The sections were not any decalcified. They were stained with Villanueva's bone staining powder and thionin dye. The microradiographs of the sections were obtained by a soft x-ray apparatus. The area, length, width, and perimeter of the perfect osteon and haversian canal were measured. The type II osteon number, osteon fragment number, and area of triangle were also determined. These parameters were examined by an image analyzer. The parameters of the osteon showed a high correlation coefficient with age (magnitude of r > or = 0.77), whereas those of the haversian canal were low (magnitude of r < or = 0.11). All parameters were subjected to multiple regression analysis for producing a multiple regression equation of age estimation. For the stepwise selection method, the perimeter of osteon, length of the haversian canal, and osteon fragment number were selected for the equation. Their multiple r2 and standard error of estimation were 0.8874 and 6.39, respectively. For the forward selection method, in addition to the above items, three parameters, the length of osteon, area of triangle, and width of osteon, were selected. Their multiple r2 and standard error of estimation were 0.9484 and 4.884, respectively. Bone staining was useful to clarify the demarcation between osteon and fragment, leading to increased accuracy of age estimation. However, precisely estimating age for the entire range from birth to 90 years was difficult.
本研究旨在通过股骨组织形态计量学估计尸体的死亡年龄。使用了来自43天至92岁的72名日本男性和2至88岁的26名女性的样本。通过用砂纸研磨将切片厚度调整至50 - 70微米。切片未进行任何脱钙处理。它们用维拉纽瓦骨染色粉和硫堇染料染色。通过软X射线设备获得切片的显微放射照片。测量了完整骨单位和哈弗斯管的面积、长度、宽度和周长。还确定了II型骨单位数量、骨单位碎片数量和三角形面积。这些参数通过图像分析仪进行检查。骨单位的参数与年龄显示出较高的相关系数（r值≥0.77），而哈弗斯管的参数则较低（r值≤0.11）。对所有参数进行多元回归分析以生成年龄估计的多元回归方程。对于逐步选择法，方程选择了骨单位周长、哈弗斯管长度和骨单位碎片数量。它们的复相关系数r²和估计标准误差分别为0.8874和6.39。对于向前选择法，除上述项目外，还选择了骨单位长度、三角形面积和骨单位宽度这三个参数。它们的复相关系数r²和估计标准误差分别为0.9484和4.884。骨染色有助于明确骨单位和碎片之间的界限，从而提高年龄估计的准确性。然而，精确估计从出生到90岁整个年龄段的年龄是困难的。
