Valdenaire O, Giller T, Breu V, Ardati A, Schweizer A, Richards J G
Pharma Division, Preclinical Research, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
FEBS Lett. 1998 Mar 13;424(3):193-6. doi: 10.1016/s0014-5793(98)00170-7.
The cloning of a cDNA encoding a G protein-coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET(B)R-LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET(B)R-LP and which was therefore termed ET(B)R-LP-2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET(B)R-LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK-293 cells stably expressing ET(B)R-LP-2 did not bind endothelin-1, endothelin-2, endothelin-3, bombesin, cholecystokinin-8 or gastrin-releasing peptide.
最近报道了一种编码与内皮素B型受体同源但不能结合内皮素的G蛋白偶联受体的cDNA的克隆，并将其命名为ET(B)R-LP。我们在此报告了一种人类cDNA的分离，该cDNA编码一种与ET(B)R-LP高度相关的受体，因此被命名为ET(B)R-LP-2。两种氨基酸序列的比较显示总体同源性为68%，一致性为48%。与ET(B)R-LP的情况一样，新受体在人类中枢神经系统中强烈表达（例如在小脑伯格曼胶质细胞、大脑皮层、内囊纤维中）。稳定表达ET(B)R-LP-2的HEK-293细胞膜不结合内皮素-1、内皮素-2、内皮素-3、蛙皮素、胆囊收缩素-8或胃泌素释放肽。
