Sohmiya M, Ishikawa K, Kato Y
Department of Medicine, Shimane Medical University, Izumo, Japan.
Eur J Endocrinol. 1998 Mar;138(3):302-6. doi: 10.1530/eje.0.1380302.
We have investigated the effect of human GH on erythropoietin (EPO) secretion in eight anemic patients with chronic renal failure (CRF) (three males and five females, aged from 46 to 83 years). Recombinant human GH was infused subcutaneously at a flow rate of 2 microg/kg body weight per 0.1 ml/h for 72 h using a portable infusion pump. Blood samples were obtained immediately before and 2, 4, 6, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120 and 168 h after the start of GH infusion. Storage urine samples were obtained before and 24, 48 and 72 h after the start of the infusion. The mean (+/- S.E.M.) basal plasma GH levels increased from 1.9 +/- 0.3 to 18.8 +/- 0.7 microg/l during the GH infusion. Plasma IGF-I levels increased 12 h after the start of GH treatment and the mean peak values of 403.6 +/- 38.5 microg/l were obtained at 72 h. Plasma EPO levels increased 6 h after the start of GH infusion, and the peak values of 38.4 +/- 11.6 IU/l were obtained at 96 h (P < 0.05 vs basal values 24.5 +/- 7.2 IU/l). Reticulocyte counts increased from 28.7 +/- 5.2 x 10(3)/microl to 40.3 +/- 8.0 x 10(3)/l at 108 h, 43.6 +/- 9.2 x 10(3)/microl at 120 h and 41.7 +/- 7.7 x 10(3)/microl at 160 h (P < 0.05). Serum urea nitrogen decreased at 72 h (P < 0.05), whereas there was no significant change in urinary excretion of nitrogen. Hemoglobin levels were not significantly changed throughout the experimental period. These findings indicate that human GH has a stimulating effect on EPO secretion in anemic patients with CRF.
我们研究了重组人生长激素（GH）对8例慢性肾功能衰竭（CRF）贫血患者促红细胞生成素（EPO）分泌的影响（3例男性和5例女性，年龄46至83岁）。使用便携式输液泵，以每0.1ml/h 2μg/kg体重的流速皮下输注重组人生长激素72小时。在开始GH输注前以及输注开始后2、4、6、12、24、36、48、60、72、84、96、108、120和168小时采集血样。在输注开始前以及输注开始后24、48和72小时采集储存尿样。在GH输注期间，平均（±标准误）基础血浆GH水平从1.9±0.3μg/L升至18.8±0.7μg/L。GH治疗开始后12小时血浆IGF-I水平升高，在72小时时获得平均峰值403.6±38.5μg/L。GH输注开始后6小时血浆EPO水平升高，在96小时时获得峰值38.4±11.6IU/L（与基础值24.5±7.2IU/L相比，P<0.05）。网织红细胞计数在108小时时从28.7±5.2×10³/μl升至40.3±8.0×10³/μl，在120小时时为43.6±9.2×10³/μl，在160小时时为41.7±7.7×10³/μl（P<0.05）。血清尿素氮在72小时时下降（P<0.05），而尿氮排泄无显著变化。在整个实验期间血红蛋白水平无显著变化。这些发现表明，重组人生长激素对CRF贫血患者的EPO分泌有刺激作用。
