Boye M, Jensen T K, Møller K, Leser T D, Jorsal S E
Danish Veterinary Laboratory, Copenhagen V.
Vet Pathol. 1998 Mar;35(2):153-6. doi: 10.1177/030098589803500212.
Fluorescent in situ hybridization targeting 16S ribosomal RNA was used for specific detection of the obligate intracellular bacterium Lawsonia intracellularis in enterocytes from pigs affected by proliferative enteropathy. A specific oligonucleotide probe was designed and the specificity of the probe was determined by simultaneous comparison with indirect immunofluorescence assay for detection of L. intracellularis in formalin-fixed tissue samples from 15 pigs affected by porcine proliferative enteropathy. We used 10 tissue samples from pigs without proliferative mucosal changes as negative controls. The results showed that the oligonucleotide probe is specific for L. intracellularis and that fluorescent in situ hybridization targeting ribosomal RNA is a suitable and fast method for specific detection and histological recognition of L. intracellularis in formalin-fixed tissue.
针对16S核糖体RNA的荧光原位杂交技术用于特异性检测受增生性肠炎影响的猪肠细胞中的专性胞内菌——胞内劳森菌。设计了一种特异性寡核苷酸探针，并通过与间接免疫荧光法同时比较，确定该探针的特异性，间接免疫荧光法用于检测来自15头受猪增生性肠炎影响的猪的福尔马林固定组织样本中的胞内劳森菌。我们使用来自无增生性黏膜变化的猪的10个组织样本作为阴性对照。结果表明，该寡核苷酸探针对胞内劳森菌具有特异性，并且针对核糖体RNA的荧光原位杂交是一种适用于福尔马林固定组织中胞内劳森菌特异性检测和组织学识别的快速方法。
