整合素β1在人牙髓细胞黏附于层粘连蛋白和纤连蛋白上的作用。

The role of integrin beta 1 in human dental pulp cell adhesion on laminin and fibronectin.

作者信息

Zhu Q, Safavi K E, Spangberg L S

机构信息

Department of Restorative Dentistry and Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Conn., USA.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998 Mar;85(3):314-8. doi: 10.1016/s1079-2104(98)90015-4.

DOI:10.1016/s1079-2104(98)90015-4

PMID:9540090

Abstract

OBJECTIVE

This study is to identify the expression of integrin beta 1 in human dental pulp cells and the role of integrin beta 1 in pulp cell adhesion on extracellular matrix protein laminin and fibronectin.

STUDY DESIGN

Immunoblot detection of integrin beta 1 in human pulp cells was with the use of monoclonal anti-beta 1 antibody. Dental pulp cell adhesion assay on extracellular matrix protein laminin and fibronectin and blocking cell adhesion was performed with monoclonal anti-beta 1 antibody.

RESULT

Integrin beta 1 was identified in human dental pulp cells. Pulp cells adhered and spread on both laminin and fibronectin. Monoclonal anti-beta 1 antibody inhibited human dental pulp cells adhesion on laminin but not on fibronectin.

CONCLUSIONS

Integrin beta 1 was expressed on human dental pulp cells and mediated cell adhesion on laminin. Human dental pulp cells also adhered on fibronectin but the adhesion was not regulated by beta 1 integrin.

摘要