整合素在人牙髓细胞中的表达及其在细胞黏附于细胞外基质蛋白上的作用。

Integrin expression in human dental pulp cells and their role in cell attachment on extracellular matrix proteins.

作者信息

Zhu Q, Safavi K E, Spångberg L S

机构信息

Department of Restorative Dentistry and Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington 06030-1715, USA.

出版信息

J Endod. 1998 Oct;24(10):641-4. doi: 10.1016/S0099-2399(98)80145-3.

DOI:10.1016/S0099-2399(98)80145-3

PMID:10023243

Abstract

Integrins are a family of heterodimeric glycoproteins consisting of alpha and beta subunits that noncovalently interact to form cell surface adhesion receptors. The objective of this study was to identify integrins in human dental pulp cells and determine their role in human dental pulp cell attachment to the biological active molecules, laminin and fibronectin. Integrin expression was studied by immunoblot and immunoprecipitation using monoclonal integrin antibodies. The role of integrin in human dental pulp cell adhesion on laminin and fibronectin was determined by inhibition of cell adhesion with those antibodies. This study found human dental pulp cells expressed alpha 1, alpha 3, alpha 5, alpha 6, alpha v, and beta 1 integrin subunits. The adhesion of human dental pulp cells to laminin and fibronectin was not inhibited by monoclonal antibody to any subunit, except that anti-beta 1 antibody inhibited pulp cells adhesion on laminin. These data provide information for further studying the role of integrins in dental pulp cell biological function.

摘要

整合素是一类由α和β亚基组成的异二聚体糖蛋白家族，这些亚基通过非共价相互作用形成细胞表面粘附受体。本研究的目的是鉴定人牙髓细胞中的整合素，并确定它们在人牙髓细胞与生物活性分子层粘连蛋白和纤连蛋白附着过程中的作用。使用单克隆整合素抗体通过免疫印迹和免疫沉淀研究整合素表达。通过用这些抗体抑制细胞粘附来确定整合素在人牙髓细胞与层粘连蛋白和纤连蛋白粘附过程中的作用。本研究发现人牙髓细胞表达α1、α3、α5、α6、αv和β1整合素亚基。除抗β1抗体抑制牙髓细胞与层粘连蛋白的粘附外，单克隆抗体对任何亚基均未抑制人牙髓细胞与层粘连蛋白和纤连蛋白的粘附。这些数据为进一步研究整合素在牙髓细胞生物学功能中的作用提供了信息。