Kumar V, Selvakumar N, Venkatesan P, Chandrasekaran V, Paramasivan C N, Prabhakar R
Tuberculosis Research Centre, Chennai.
Indian J Med Res. 1998 Feb;107:75-7.
Twenty three clinical isolates M. tuberculosis and the reference strain, M. tuberculosis H37Rv were tested for their susceptibility to trifluoperazine (TFP) by the standard broth dilution method and the bioluminescence assay. The results showed that in 15 of the 23 isolates, the minimal inhibitory concentration (MIC) was identical in both the methods and in the remaining 8 isolates the difference in the MIC values between the methods, was less than two fold and was not significant. The findings suggest that the measurement of adenosine triphosphate (ATP) by bioluminescence assay can be employed as an alternative method for the rapid screening of clinical isolates for their susceptibility to anti-mycobacterial agents.
采用标准肉汤稀释法和生物发光测定法，对23株临床分离的结核分枝杆菌以及参考菌株结核分枝杆菌H37Rv进行了三氟拉嗪（TFP）敏感性测试。结果显示，在23株分离菌株中，有15株的最低抑菌浓度（MIC）在两种方法中相同，其余8株中两种方法的MIC值差异小于两倍，且不显著。这些结果表明，通过生物发光测定法测量三磷酸腺苷（ATP），可作为一种替代方法，用于快速筛查临床分离菌株对抗分枝杆菌药物的敏感性。
