Tsai C C, Hong Y C, Chen C C, Wu Y M
School of Dentistry, Kaohsiung Medical College, Taiwan.
J Dent. 1998 Mar;26(2):97-103. doi: 10.1016/s0300-5712(96)00084-x.
The arachidonic acid metabolites prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) are inflammatory mediators which are likely to be involved in the pathogenesis of periodontal disease. PGE2 mediates vasodilatation, increases vascular permeability, enhances pain perception by bradykinin and histamine, alters connective tissue metabolism and enhances osteoclastic bone resorption. LTB4 causes the accumulation of inflammatory cells in the inflamed sites, and degranulation of polymorphonuclear leukocytes.
To measure gingival crevicular fluid (GCF) levels of PGE2, LTB4 and periodontal health.
The periodontal condition of 24 subjects was evaluated on the basis of plaque index, gingival index, probing depth, and attachment level. GCF samples were collected from one or two site(s) of each sextant per subject and the volume was measured using Periotron 6000. Samples were then assayed for PGE2 and LTB4 using a competitive enzyme immunoassay. Mean PGE2 and LTB4 levels were determined for each subject and group means compared.
Significant differences in the levels of PGE2 and LTB4 were found between patients with periodontitis, and non-periodontitis individuals (P < 0.001). The PGE2/LTB4 levels were positively correlated with the clinical parameters (P < 0.01) and reduced markedly after phase 1 of the periodontal treatment (P < 0.01). The total amount and concentration (ng ml-1) of LTB4 was positively correlated with the gingival index (P < 0.01).
These results indicate that the levels of PGE2 correlated with the severity of the periodontal status, and the levels of LTB4 correlated with gingival inflammation. Thus, our data suggest that the total amounts of PGE2/LTB4 may be good indicators for periodontal inflammation.
花生四烯酸代谢产物前列腺素E2（PGE2）和白三烯B4（LTB4）是炎症介质，可能参与牙周病的发病机制。PGE2介导血管舒张，增加血管通透性，通过缓激肽和组胺增强疼痛感知，改变结缔组织代谢并增强破骨细胞的骨吸收。LTB4导致炎症部位炎症细胞积聚以及多形核白细胞脱颗粒。
测量龈沟液（GCF）中PGE2、LTB4水平及牙周健康状况。
根据菌斑指数、牙龈指数、探诊深度和附着水平对24名受试者的牙周状况进行评估。从每位受试者每个牙象限的一个或两个部位采集GCF样本，并使用Periotron 6000测量其体积。然后使用竞争性酶免疫测定法检测样本中的PGE2和LTB4。确定每位受试者的PGE2和LTB4平均水平，并比较组均值。
牙周炎患者与非牙周炎个体之间PGE2和LTB4水平存在显著差异（P < 0.001）。PGE2/LTB4水平与临床参数呈正相关（P < 0.01），在牙周治疗第一阶段后显著降低（P < 0.01）。LTB4的总量和浓度（ng/ml）与牙龈指数呈正相关（P < 0.01）。
这些结果表明PGE2水平与牙周状况的严重程度相关，LTB4水平与牙龈炎症相关。因此，我们的数据表明PGE2/LTB4的总量可能是牙周炎症的良好指标。
