Emingil G, Cinarcik S, Baylas H, Coker I, Hüseyinov A
Ege University, School of Dentistry, Department of Periodontology, Izmir, Turkey.
J Periodontol. 2001 Aug;72(8):1025-31. doi: 10.1902/jop.2001.72.8.1025.
Leukotriene B4 (LTB4), a product of the lipoxygenase pathway of arachidonic acid metabolism, exhibits numerous activities that can account for most of the features of host responses seen in periodontal diseases. The aim of the present study was to examine the role of LTB4 in the pathogenesis of specific periodontal diseases.
LTB4 levels were investigated in gingival crevicular fluid (GCF) and gingival tissue (GT) samples of 10 patients with chronic periodontitis (CP), 12 patients with generalized aggressive periodontitis (GAgP), 6 patients with localized aggressive periodontitis (LAgP), 6 patients with gingivitis (G), and 6 periodontally healthy subjects (H). Periodontal status was evaluated by measuring probing depth, gingival index, papillary bleeding index, and plaque index. LTB4 was extracted from the samples by solid-phase method using C18 cartridge and was purified by high performance liquid chromatographic method and then analyzed by radioimmunoassay.
All patient groups had significantly higher levels of GCF and GT LTB4 compared to the control group (P<0.005). The CP patients had the highest LTB4 levels compared to those in other patient groups (P<0.005). GAgP, LAgP, and G groups had similar amounts of GCF and GT LTB4 (P>0.005). When the data were expressed as concentration, the CP group was found to have higher concentration of LTB4, compared to that of control group (P<0.005). GAgP, LAgP, and G groups had similar LTB4 concentration compared to that of control group (P>0.005). No significant difference was found between GAgP, LAgP, and G groups (P>0.005). The CP group had higher LTB4 concentration compared to both GAgP and LAgP groups (P<0.005). Although the CP group had a higher GCF LTB4 concentration compared to G group, this difference did not reach significance (P>0.005). No significant correlation was found between GCF and GT LTB4 levels and clinical parameters.
The results of the present study indicate that LTB4 is likely to be an important mediator in regulating inflammatory responses in the human periodontal tissues. This lipid mediator may play an important role in the pathophysiology of periodontal disease.
白三烯B4（LTB4）是花生四烯酸代谢脂氧合酶途径的产物，具有多种活性，可解释牙周疾病中宿主反应的大多数特征。本研究的目的是探讨LTB4在特定牙周疾病发病机制中的作用。
对10例慢性牙周炎（CP）患者、12例广泛侵袭性牙周炎（GAgP）患者、6例局限性侵袭性牙周炎（LAgP）患者、6例牙龈炎（G）患者和6例牙周健康受试者（H）的龈沟液（GCF）和牙龈组织（GT）样本中的LTB4水平进行了研究。通过测量探诊深度、牙龈指数、龈乳头出血指数和菌斑指数来评估牙周状况。使用C18柱通过固相法从样本中提取LTB4，并用高效液相色谱法纯化，然后通过放射免疫测定法进行分析。
与对照组相比，所有患者组的GCF和GT LTB4水平均显著更高（P<0.005）。与其他患者组相比，CP患者的LTB4水平最高（P<0.005）。GAgP、LAgP和G组的GCF和GT LTB4含量相似（P>0.005）。当数据以浓度表示时，发现CP组的LTB4浓度高于对照组（P<0.005）。与对照组相比，GAgP、LAgP和G组的LTB4浓度相似（P>0.005）。GAgP、LAgP和G组之间未发现显著差异（P>0.005）。CP组的LTB4浓度高于GAgP组和LAgP组（P<0.005）。尽管CP组的GCF LTB4浓度高于G组，但这种差异未达到显著水平（P>0.005）。GCF和GT LTB4水平与临床参数之间未发现显著相关性。
本研究结果表明，LTB4可能是调节人类牙周组织炎症反应的重要介质。这种脂质介质可能在牙周疾病的病理生理学中起重要作用。
