编码人神经胰蛋白酶的cDNA的克隆与测序
Cloning and sequencing of the cDNA encoding human neurotrypsin.
作者信息
Proba K, Gschwend T P, Sonderegger P
机构信息
Institute of Biochemistry, University of Zurich, Switzerland.
出版信息
Biochim Biophys Acta. 1998 Mar 9;1396(2):143-7. doi: 10.1016/s0167-4781(97)00205-4.
PMID:9540828
Abstract
cDNA clones encoding human neurotrypsin have been isolated from a human fetal brain cDNA library using a PCR-amplified probe. The assembled cDNA sequence contains a 2625 bp open reading frame encoding a multidomain serine protease with an overall sequence identity of 82.5% to murine neurotrypsin. Surprisingly, the human neurotrypsin exhibits an additional scavenger receptor cysteine-rich repeat.
摘要
使用聚合酶链反应（PCR）扩增的探针，已从人胎脑cDNA文库中分离出编码人神经胰蛋白酶的cDNA克隆。组装后的cDNA序列包含一个2625 bp的开放阅读框，编码一种多结构域丝氨酸蛋白酶，其与小鼠神经胰蛋白酶的总体序列同一性为82.5%。令人惊讶的是，人神经胰蛋白酶还具有一个额外的富含半胱氨酸的清道夫受体重复序列。
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