Loshon C A, Beary K E, Gouveia K, Grey E Z, Santiago-Lara L M, Setlow P
Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
Biochim Biophys Acta. 1998 Mar 9;1396(2):148-52. doi: 10.1016/s0167-4781(97)00204-2.
The single sspE genes coding for gamma-type small, acid-soluble spore proteins (SASP) of three round-spore-forming bacteria, Bacillus aminovorans, Sporosarcina halophila and S. ureae, have been cloned and sequenced. While the deduced amino acid sequences of these three gamma-type SASP show clear homology to those from six Bacillus species that do not form round spores, there are no residues conserved completely among the 9 sequences known. In addition, the 139 residue B. aminovorans protein is 35 residues larger than any other while the 60 residue S. halophila protein is one of the smallest. These data suggest that the sspE genes have been under little selective pressure in recent evolutionary time.
编码三种圆形芽孢形成细菌——氨基食芽孢杆菌、嗜盐芽孢八叠球菌和尿素芽孢八叠球菌的γ型小酸溶性芽孢蛋白(SASP)的单个sspE基因已被克隆和测序。虽然这三种γ型SASP的推导氨基酸序列与六种不形成圆形芽孢的芽孢杆菌属物种的序列有明显的同源性,但在已知的9个序列中没有完全保守的残基。此外,含有139个残基的氨基食芽孢杆菌蛋白比其他任何蛋白都大35个残基,而含有60个残基的嗜盐芽孢八叠球菌蛋白是最小的蛋白之一。这些数据表明,sspE基因在最近的进化时间里几乎没有受到选择压力。