Damiani E, Sugiyama T, Shimamura K, Greci L, Matsuda Y
Dipartimento di Scienze dei Materiali e della Terra, Università, Ancona, Italy.
FEBS Lett. 1998 Mar 20;425(1):123-5. doi: 10.1016/s0014-5793(98)00212-9.
The expression of the contractile proteins, alpha-actin, smooth muscle myosin heavy chain-1 (SM1) and calponin present in smooth muscle cells (SMC) in the presence of oxidized low density lipoproteins (oxLDL) was investigated in two different cell cultures: the mouse smooth muscle cell line SVSC and rat smooth muscle cells (RSMC). Exposure of the cells to 187 microg protein/ml oxLDL for 24 h reduced the expression of all three contractile proteins in both cell cultures when compared to cells incubated in the presence of native LDL. This investigation of the response of SMC contractile proteins to oxLDL may provide further insights into the mechanisms by which oxidatively modified LDL is atherogenic and suggests that oxLDL may contribute to the regulation of the expression of the genes responsible for the synthesis of smooth muscle cell contractile proteins.
在两种不同的细胞培养物中，即小鼠平滑肌细胞系SVSC和大鼠平滑肌细胞（RSMC）中，研究了在氧化型低密度脂蛋白（oxLDL）存在的情况下，平滑肌细胞（SMC）中收缩蛋白α-肌动蛋白、平滑肌肌球蛋白重链-1（SM1）和钙调蛋白的表达。与在天然低密度脂蛋白存在下孵育的细胞相比，将细胞暴露于187微克蛋白质/毫升的oxLDL中24小时，会降低两种细胞培养物中所有三种收缩蛋白的表达。对SMC收缩蛋白对oxLDL反应的这项研究，可能会为氧化修饰的低密度脂蛋白致动脉粥样硬化的机制提供进一步的见解，并表明oxLDL可能有助于调节负责平滑肌细胞收缩蛋白合成的基因的表达。
