Webb P P, Moxham B J, Ralphs J R, Benjamin M
Anatomy Unit, School of Molecular and Medical Biosciences, University of Wales, Cardiff, UK.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:147-55. doi: 10.1111/j.1600-0722.1998.tb02168.x.
This study identifies different types of collagens during tooth development, maturation and ageing. Tissues from the rat first molar (from animals ranging in age from E14 to 104 wk postnatally) were immunostained using a panel of mono- and polyclonal antibodies against types I, II, III, IV, VI, IX and X collagen, fibronectin and laminin. During tooth development, types I and III collagens were expressed in the dental papilla at all stages but were also unexpectedly observed in the stellate reticulum of the enamel organ. Transient expression of type II collagen was also observed in the stellate reticulum during the late bell stage. Types IV and VI collagens, with laminin and fibronectin, were located within the basement membranes of the tooth germ. Collagen types I and III were observed within the developing follicle/periodontal ligament, type III predominating where collagen fibres were inserting into the alveolar bone and cementum. The pattern of types I and III collagen labelling within the periodontal ligament and the dental pulp did not change with age. Thus, some unusual collagen localisations were observed in the tooth germ, particularly within the stellate reticulum.
本研究鉴定了牙齿发育、成熟和衰老过程中的不同类型胶原蛋白。使用一组针对I型、II型、III型、IV型、VI型、IX型和X型胶原蛋白、纤连蛋白和层粘连蛋白的单克隆和多克隆抗体，对大鼠第一磨牙（来自出生后14天至104周龄的动物）的组织进行免疫染色。在牙齿发育过程中，I型和III型胶原蛋白在牙乳头的所有阶段均有表达，但在釉质器官的星网状层中也意外观察到。在钟状期末期，星网状层中也观察到II型胶原蛋白的短暂表达。IV型和VI型胶原蛋白与层粘连蛋白和纤连蛋白一起位于牙胚的基底膜内。在发育中的滤泡/牙周韧带中观察到I型和III型胶原蛋白，III型在胶原纤维插入牙槽骨和牙骨质的部位占主导。牙周韧带和牙髓内I型和III型胶原蛋白的标记模式不随年龄变化。因此，在牙胚中观察到一些不寻常的胶原蛋白定位，特别是在星网状层内。
