Moxham B J, Webb P P, Benjamin M, Ralphs J R
Anatomy Unit, School of Molecular and Medical Biosciences, University of Wales Cardiff, UK.
Eur J Oral Sci. 1998 Jan;106 Suppl 1:376-83. doi: 10.1111/j.1600-0722.1998.tb02201.x.
The periodontal ligament and dental pulp connective tissues are foetal-like, and their process of ageing may therefore differ from other tissues of mesenchymal origin. Several studies have already highlighted the lack of age changes in the extracellular matrix (ECM), but more needs to be known about cellular changes. For this study, tissues from the first molar teeth of Wistar rats aged 12 wk and 104 wk were compared by immunolocalisation of cytoskeletal components. Tissues from the first molar were immunostained, employing a panel of 16 monoclonal antibodies against cytokeratins, vimentin, F-actin and tubulin. Within the pulp, labelling for vimentin in both odontoblasts and pulpal fibroblasts and F-actin in the cell processes of odontoblasts was detected at both ages but with marked reduction in labelling in the older tissue. Within the periodontal ligament, vimentin labelling was weaker in the aged tissue, especially nearer the cementum. More significantly, the fibroblasts of the aged periodontal ligament expressed cytokeratin 19. In contrast to reports of little age change to the ECM, the cells of the pulp, and particularly the periodontal ligament, show marked changes to their cytoskeletal components.
牙周韧带和牙髓结缔组织呈胎儿样，因此它们的老化过程可能与其他间充质来源的组织不同。一些研究已经强调了细胞外基质（ECM）缺乏年龄变化，但关于细胞变化还需要了解更多。在本研究中，通过细胞骨架成分的免疫定位比较了12周龄和104周龄Wistar大鼠第一磨牙的组织。使用一组针对细胞角蛋白、波形蛋白、F-肌动蛋白和微管蛋白的16种单克隆抗体对第一磨牙的组织进行免疫染色。在牙髓内，在两个年龄段的成牙本质细胞和牙髓成纤维细胞中均检测到波形蛋白的标记，在成牙本质细胞的细胞突起中检测到F-肌动蛋白的标记，但在较老的组织中标记明显减少。在牙周韧带内，老年组织中波形蛋白的标记较弱，尤其是靠近牙骨质的地方。更显著的是，老年牙周韧带的成纤维细胞表达细胞角蛋白19。与关于ECM几乎没有年龄变化的报道相反，牙髓细胞，尤其是牙周韧带细胞，其细胞骨架成分显示出明显变化。
