Spergel J M, Mizoguchi E, Brewer J P, Martin T R, Bhan A K, Geha R S
Division of Immunology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
J Clin Invest. 1998 Apr 15;101(8):1614-22. doi: 10.1172/JCI1647.
Our understanding of the pathogenesis of atopic dermatitis (AD) and its relationship to asthma remains incomplete. Herein, we describe a murine model of epicutaneous (EC) sensitization to the protein allergen, chicken egg albumin, ovalbumin (OVA), which results in a rise in total and OVA-specific serum IgE and leads to the development of a dermatitis characterized by infiltration of CD3(+) T cells, eosinophils, and neutrophils and by local expression of mRNA for the cytokines IL-4, IL-5, and interferon-gamma. A single exposure of the EC sensitized mice to aerosolized OVA induced eosinophilia in the bronchoalveolar lavage fluid and airway hyperresponsiveness to intravenous methacholine as assessed by measurement of pulmonary dynamic compliance (Cdyn). These results suggest a possible role for EC exposure to antigen in atopic dermatitis and in the development of allergic asthma.
我们对特应性皮炎（AD）的发病机制及其与哮喘的关系的理解仍不完整。在此，我们描述了一种对蛋白质过敏原鸡卵清蛋白（OVA）进行表皮（EC）致敏的小鼠模型，该模型导致总血清IgE和OVA特异性血清IgE升高，并引发一种皮炎，其特征为CD3(+) T细胞、嗜酸性粒细胞和中性粒细胞浸润，以及细胞因子IL-4、IL-5和干扰素-γ的mRNA在局部表达。通过测量肺动态顺应性（Cdyn）评估，将经EC致敏的小鼠单次暴露于雾化OVA会诱导支气管肺泡灌洗液中的嗜酸性粒细胞增多以及气道对静脉注射乙酰甲胆碱的高反应性。这些结果表明，EC暴露于抗原在特应性皮炎和过敏性哮喘的发展中可能起作用。
