Martínez M E, Medina S, del Campo M T, Sánchez-Cabezudo M J, Sánchez M, Munuera L
Clinical Biochemistry Division, Hospital La Paz, Pso. de la Castellana 261, 28046 Madrid, Spain.
Calcif Tissue Int. 1998 May;62(5):453-6. doi: 10.1007/s002239900459.
We have studied the direct effects of polyethylene particles on osteoblastic function in primary human bone cell cultures. The cells were obtained from trabecular bone fragments of patients undergoing knee reconstructive surgery. When the cells reached confluency, they were subcultured into two flasks, one untreated (control culture) and the other treated with polyethylene particles, and incubated until confluency. Osteoblastic function was evaluated by assaying osteocalcin, alkaline phosphatase, and C-terminal procollagen type I, with and without 1,25(OH)2D stimulation, in the cell-conditioned medium. We found that addition of polyethylene to these osteoblastic cell cultures induced higher levels of secreted osteocalcin after 1, 25(OH)2D stimulation. Alkaline phosphatase levels increased whereas C-terminal procollagen type I levels decreased in the cell conditioned medium after polyethylene was added to the cultures. Treatment of the control cultures with 1,25(OH)2D stimulated alkaline phosphatase levels and decreased C-terminal procollagen type I. However, these osteoblastic markers in 1,25(OH)2D-treated cells did not change in cultures with polyethylene. This study demonstrates that polyethylene particles have a direct effect on osteoblastic markers in human bone cells in culture.
我们研究了聚乙烯颗粒对原代人骨细胞培养中骨细胞功能的直接影响。细胞取自接受膝关节重建手术患者的小梁骨碎片。当细胞达到汇合状态时，将其传代培养到两个培养瓶中，一个未处理（对照培养），另一个用聚乙烯颗粒处理，然后孵育至汇合。通过检测细胞条件培养基中骨钙素、碱性磷酸酶和I型前胶原C端，在有无1,25(OH)2D刺激的情况下评估骨细胞功能。我们发现，在这些骨细胞培养物中添加聚乙烯后，1,25(OH)2D刺激后分泌的骨钙素水平更高。添加聚乙烯后，细胞条件培养基中的碱性磷酸酶水平升高，而I型前胶原C端水平降低。用1,25(OH)2D处理对照培养物可刺激碱性磷酸酶水平并降低I型前胶原C端。然而，在添加聚乙烯的培养物中，1,25(OH)2D处理的细胞中的这些骨细胞标志物没有变化。这项研究表明，聚乙烯颗粒对培养的人骨细胞中的骨细胞标志物有直接影响。
