Martínez M E, del Campo M T, Medina S, Sánchez M, Sánchez-Cabezudo M J, Esbrit P, Martínez P, Moreno I, Rodrigo A, Garcés M V, Munuera L
Biochemistry Division, Hospital La Paz, Pso. de la Castellana, 261, 28046, Madrid, Spain.
Calcif Tissue Int. 1999 Apr;64(4):280-6. doi: 10.1007/s002239900619.
Bone loss with aging may be due, at least in part, to inadequate bone formation. Moreover, the process of bone aging is known to follow a different pattern throughout the skeleton. In this study, we examined the cell proliferation rate (area under the cell growth curve, AUC) and the secretion of C-terminal type I procollagen (PICP), alkaline phosphatase (ALP), and osteocalcin (OC) in primary cultures of osteoblastic cells from human trabecular bone. Osteoblastic cells were obtained for 168 donors (100 women and 68 men). Ninety-eight bone samples were obtained from subjects undergoing knee arthroplastia, 52 aged 50-70 years (64 +/- 5) and 46 over age 70 (73 +/- 2). Another 70 bone samples were obtained from subjects undergoing hip arthroplastia; 51 were 50-70 years old (64 +/- 4) and 19 were over 70 (75 +/- 5). Osteoblastic cells from the older donors had a lower proliferation rate and OC secretion than those from younger subjects. However, ALP secretion was higher in the former subjects, whereas PICP secretion was unchanged. Osteoblastic cells from hip had a lower proliferation rate than those from knee. PICP secretion was also lower and ALP secretion was higher in the former cells. In age-matched cell cultures, osteoblastic cells from the knee had higher proliferation rate and PICP secretion than osteoblastic cells from the hip. However, ALP secretion was lower in knee osteoblastic cells than those from hip only in the younger group. With aging, ALP secretion was found to increase in knee osteoblactic cells, whereas OC secretion decreased in osteoblastic cell cultures from the hip. Our findings suggest that bone loss with aging may be accounted for, at least in part, by a decreased osteoblastic cell proliferation and an increased osteoblastic maturation. In addition, our data indicate that these changes with aging do not occur similarly at different skeletal sites.
随着年龄增长出现的骨质流失可能至少部分归因于骨形成不足。此外，已知整个骨骼的骨老化过程遵循不同的模式。在本研究中，我们检测了来自人小梁骨的成骨细胞原代培养物中的细胞增殖率（细胞生长曲线下面积，AUC）以及I型前胶原C端（PICP）、碱性磷酸酶（ALP）和骨钙素（OC）的分泌情况。从168名供体（100名女性和68名男性）获取成骨细胞。从接受膝关节置换术的受试者中获取98块骨样本，52名年龄在50 - 70岁（64±5岁），46名年龄超过70岁（73±2岁）。另外70块骨样本从接受髋关节置换术的受试者中获取；51名年龄在50 - 70岁（64±4岁），19名年龄超过70岁（75±5岁）。来自老年供体的成骨细胞增殖率和OC分泌低于年轻受试者的成骨细胞。然而，前者的ALP分泌较高，而PICP分泌没有变化。来自髋关节的成骨细胞增殖率低于来自膝关节的成骨细胞。前者细胞中的PICP分泌也较低，而ALP分泌较高。在年龄匹配的细胞培养物中，来自膝关节的成骨细胞增殖率和PICP分泌高于来自髋关节的成骨细胞。然而，仅在较年轻组中，膝关节成骨细胞的ALP分泌低于髋关节成骨细胞。随着年龄增长，发现膝关节成骨细胞中的ALP分泌增加，而髋关节成骨细胞培养物中的OC分泌减少。我们的研究结果表明，随着年龄增长出现的骨质流失可能至少部分是由于成骨细胞增殖减少和成骨细胞成熟增加所致。此外，我们的数据表明，这些随年龄增长的变化在不同骨骼部位并非以相同方式发生。
