Gollop R, Nakagomi O, Silberstein I, Shulman L M, Greenberg H B, Mendelson E, Shif I
Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
Arch Virol. 1998;143(2):263-77. doi: 10.1007/s007050050285.
Insight into the origin of human rotaviruses carrying the AU-1 VP4 allele was gained by examining their genomic RNA constellation using RNA-RNA hybridization and by sequencing the VP8* portion (nucleotides 1-750) of their gene 4. AU-1 like viruses isolated in Israel from children attending outpatient clinics were classified into three sub-genogroups based on RNA-RNA hybridization analysis: Subgenogroup 1 consists of two strains (Ro-5829 and Ro-5960) which belong to the AU-1 genogroup, since all their 11 segments hybridized to AU-1 segments. Subgenogroup 2 consists of one reassortant virus (Ro-5193) of which seven RNA segments hybridized to AU-1 segments and the remaining four segments hybridized to NCDV (bovine rotavirus). Subgenogroup 3 consists of four reassortant viruses (Ro-6460, Ro-6584, Ro-6784 and Ro-7044) which had a common genome constellation: only four of their RNA segments hybridized to AU-1 and the other seven segments hybridized to NCDV segments. Sequence analysis of the VP8* gene also revealed a three level pattern of homology with the AU-1 prototype and the local AU-1 like strains which was consistent with the overall genomic (RNA-RNA) constellation: Subgenogroup 1 had 98-98.1% homology with the AU-1 prototype; Subgenogroup 2 had 96.8% homology with the AU-1 prototype and 95.6-96.7% homology with Subgenogroup 1; Subgenogroup 3 had 95.3-95.6% homology with the prototype AU-1 and 93.4-94.3% homology with Subgenogroup 1. Possible evolutionary pathways are discussed.
通过使用RNA - RNA杂交技术检测其基因组RNA构成，并对其基因4的VP8部分（核苷酸1 - 750）进行测序，深入了解了携带AU - 1 VP4等位基因的人轮状病毒的起源。在以色列从门诊儿童中分离出的类AU - 1病毒，根据RNA - RNA杂交分析被分为三个亚基因组群：亚基因组群1由两个菌株（Ro - 5829和Ro - 5960）组成，它们属于AU - 1基因组群，因为它们的所有11个片段都与AU - 1片段杂交。亚基因组群2由一种重配病毒（Ro - 5193）组成，其中七个RNA片段与AU - 1片段杂交，其余四个片段与NCDV（牛轮状病毒）杂交。亚基因组群3由四种重配病毒（Ro - 6460、Ro - 6584、Ro - 6784和Ro - 7044）组成，它们具有共同的基因组构成：只有四个RNA片段与AU - 1杂交，其他七个片段与NCDV片段杂交。VP8基因的序列分析还揭示了与AU - 1原型和当地类AU - 1菌株的三级同源模式，这与整体基因组（RNA - RNA）构成一致：亚基因组群1与AU - 1原型的同源性为98 - 98.1%；亚基因组群2与AU - 1原型的同源性为96.8%，与亚基因组群1的同源性为95.6 - 96.7%；亚基因组群3与原型AU - 1的同源性为95.3 - 95.6%，与亚基因组群1的同源性为93.4 - 94.3%。文中讨论了可能的进化途径。
