Mauck J C
Clin Biochem. 1976 Aug;9(4):188-91. doi: 10.1016/s0009-9120(76)80054-9.
A method is presented for the quantitation of urinary and CSF protein. In the presence of 0.10 N NaOH the peptide bonds of the protein remove and bind copper from an ion exchange resin. The resulting copper-protein complex is separated from low molecular weight substances by gel filtration and the copper in the eluted complex is determined colorimetrically with diethyldithiocarbamic acid. The method requires only 100mul of sample, has biuret specificity and uses a single prepacked column. The limit of sensitivity is 2 mg of protein per deciliter.
本文介绍了一种定量测定尿液和脑脊液蛋白质的方法。在0.10 N氢氧化钠存在的情况下，蛋白质的肽键从离子交换树脂中去除并结合铜。通过凝胶过滤将所得的铜 - 蛋白质复合物与低分子量物质分离，并用二乙基二硫代氨基甲酸比色法测定洗脱复合物中的铜。该方法仅需100微升样品，具有双缩脲特异性，且使用单个预装柱。灵敏度极限为每分升2毫克蛋白质。
