Peele J D, Gadsden R H, Loadholt C B
Clin Chem. 1977 Jan;23(1):86-8.
We compared an immunochemical method specific for plasma proteins with a chemical method, in which interfering substances are separated by gel filtration and "total" urinary protein is determined by the biuret reaction followed by reaction of protein-bound copper with diethyldithiocarbamate after a second gel filtration to remove nonprotein-bound copper. More than 250 24-h urine samples were analyzed by each method. There was linear agreement and a correlation of 0.96 between the two methods, but urinary protein values determined by the immunochemical method, especially for patients with multiple myeloma, were lower than by the chemical method.
我们将一种针对血浆蛋白的免疫化学方法与一种化学方法进行了比较。在化学方法中，干扰物质通过凝胶过滤分离，“总”尿蛋白通过双缩脲反应测定，然后在第二次凝胶过滤以去除未结合蛋白的铜之后，使结合蛋白的铜与二乙基二硫代氨基甲酸盐反应。每种方法分析了超过250份24小时尿液样本。两种方法之间存在线性一致性，相关性为0.96，但免疫化学方法测定的尿蛋白值，尤其是对多发性骨髓瘤患者而言，低于化学方法测定的值。
