Rawadi G, Dujeancourt-Henry A, Lemercier B, Roulland-Dussoix D
Institut Pasteur, Département de Bactériologie et de Mycologie, Paris, France.
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:305-9. doi: 10.1099/00207713-48-1-305.
The nucleotide sequence of the 16S rRNA genes of four rare human mycoplasma species, Mycoplasma faucium, M. buccale, M. primatum and M. spermatophilum, were partially sequenced and compared to published rRNA genes of mycoplasmas to determine their position in the Mollicutes phylogenetic tree. Nucleotide sequence motif and overall similarities allowed positioning of these mycoplasmas in the hominis phylogenetic group, as defined by Weisburg et al. [Weisburg, W. G., Tully, J. G., Rose, D. L. & 9 other authors (1989). J Bacteriol 171, 6455-6467]. Furthermore, these mycoplasmas could be clustered into two different subdivisions of the hominis group: (i) M. faucium and M. buccale were found to be included in the M. fermentans subdivision, and (ii) M. primatum and M. spermatophilum were included in the M. hominis one. Variable regions of the 16S rRNA genes were used to determine specific PCR primers to detect and identify M. faucium.
对四种罕见的人类支原体物种，即咽支原体（Mycoplasma faucium）、颊支原体（M. buccale）、灵长类支原体（M. primatum）和嗜精支原体（M. spermatophilum）的16S rRNA基因的核苷酸序列进行了部分测序，并与已发表的支原体rRNA基因进行比较，以确定它们在柔膜菌纲系统发育树中的位置。核苷酸序列基序和总体相似性使这些支原体能够定位在由魏斯堡等人定义的人型支原体系统发育组中[魏斯堡，W.G.，塔利，J.G.，罗斯，D.L.及其他9位作者（1989年）。《细菌学杂志》171，6455 - 6467]。此外，这些支原体可聚类为人型支原体组的两个不同亚组：（i）发现咽支原体和颊支原体包含在发酵支原体亚组中，以及（ii）灵长类支原体和嗜精支原体包含在人型支原体亚组中。利用16S rRNA基因的可变区来确定特异性PCR引物，以检测和鉴定咽支原体。
