Brown D R, Crenshaw B C, McLaughlin G S, Schumacher I M, McKenna C E, Klein P A, Jacobson E R, Brown M B
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
Int J Syst Bacteriol. 1995 Apr;45(2):348-50. doi: 10.1099/00207713-45-2-348.
The nucleotide sequences of the 16S rRNA genes of two mycoplasmas, Mycoplasma agassizii (proposed sp. nov.) and Mycoplasma testudinis, isolated from tortoises were determined and used for taxonomic comparisons. Signature nucleotide sequence motifs and overall sequence similarities to other mollicutes positioned these mycoplasmas in the M. hyorhinis and M. pneumoniae phylogenetic groups, respectively. A third, previously unrecognized tortoise mycoplasma was detected by 16S rRNA gene amplification and sequence analysis and was positioned in the M. fermentans phylogenetic group. The 16S rRNA gene of Acholeplasma laidlawii was similarly detected in a tortoise isolate, showing that diverse mollicutes can share the same family of reptilian host.
测定了从乌龟分离出的两种支原体——阿加西支原体（提议的新种）和龟支原体16S rRNA基因的核苷酸序列，并用于分类比较。标志性核苷酸序列基序以及与其他柔膜菌的整体序列相似性分别将这些支原体定位在猪鼻支原体和肺炎支原体系统发育组中。通过16S rRNA基因扩增和序列分析检测到第三种先前未被识别的乌龟支原体，并将其定位在发酵支原体系统发育组中。在一株乌龟分离物中同样检测到了莱氏无胆甾原体的16S rRNA基因，表明不同的柔膜菌可以共享同一类爬行动物宿主。
