McDowall R D, Doyle E, Murkitt G S, Picot V S
Department of Drug Analysis, Smith Kline and French Research Ltd., Welwyn, Hertfordshire, UK.
J Pharm Biomed Anal. 1989;7(9):1087-96. doi: 10.1016/0731-7085(89)80047-0.
Sample preparation for the analysis of drugs in biological fluids consists of a number of unit operations that are used for (i) release of the drug from a conjugate or biological matrix; (ii) removal of endogenous compounds that could interfere with the assay; and (iii) techniques for liquid handling. The trends in sample preparation that have occurred over the past 10 years in the authors' laboratory are discussed. In general, there has been a move from the traditional liquid-liquid extraction to methods using bonded-silica which permit rapid throughput and efficient extraction. Automation of sample preparation has seen further gains in productivity; however, the present generation of equipment lack the control and communication systems that are essential for the development of the automated integrated laboratory of the future.
生物流体中药物分析的样品制备包括许多单元操作，这些操作用于：（i）从缀合物或生物基质中释放药物；（ii）去除可能干扰测定的内源性化合物；以及（iii）液体处理技术。本文讨论了作者实验室在过去10年中样品制备方面的发展趋势。总体而言，已从传统的液-液萃取转向使用键合硅胶的方法，这种方法能够实现高通量和高效萃取。样品制备的自动化在提高生产率方面取得了进一步进展；然而，目前的设备缺乏未来自动化集成实验室发展所必需的控制和通信系统。
