Wu W S, Tsai J L
Graduate Institute of Occupational Safety and Health, Kaohsiung Medical College, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1998 Feb;14(2):76-80.
Qualitative and quantitative detection of paraquat and diquat in urine was performed by capillary zone electrophoresis (CZE). In non-extracted urine, these two closely related herbicides were hydrodynamically or electrokinetically injected into the capillary and well separated in a acidic (pH 3.0) buffer (10 mM glycin, 50 mM NaCl and 20% methanol) within 8 min. The analytes can be identified by their respective migration time with no interfering endogeous compounds around. The peaks were further confirmed by their respective multi-wavelength scanning spectra. Limits of detection (LOD) were 0.2 and 0.1 mg/ml for PQ and DQ, respectively, in nonextracted urine using hydrodynamic injection. Detection sensitivity was greatly improved by solid phase extraction of the compounds followed by CZE using electro-injection under which LODs were 15 and 8 ng/ml for paraquat and diquat respectively. We conclude CZE is a useful method for laboratory diagnosis of herbicide intoxication.
采用毛细管区带电泳（CZE）对尿液中的百草枯和敌草快进行定性和定量检测。在未经萃取的尿液中，这两种密切相关的除草剂通过流体动力学或电动方式注入毛细管，并在酸性（pH 3.0）缓冲液（10 mM甘氨酸、50 mM氯化钠和20%甲醇）中于8分钟内实现良好分离。通过各自的迁移时间可识别分析物，周围无干扰性内源性化合物。通过各自的多波长扫描光谱进一步确认峰。使用流体动力学进样时，未经萃取的尿液中百草枯和敌草快的检测限（LOD）分别为0.2和0.1 mg/ml。通过对化合物进行固相萃取，然后采用电动进样的CZE方法，检测灵敏度大大提高，在此条件下，百草枯和敌草快的LOD分别为15和8 ng/ml。我们得出结论，CZE是一种用于除草剂中毒实验室诊断的有用方法。
