通过测量T细胞活化的CD69特异性细胞荧光测定法检测葡萄球菌超抗原毒素
Detection of staphylococcal superantigenic toxins by a CD69-specific cytofluorimetric assay measuring T-cell activation.
作者信息
Lina G, Cozon G, Ferrandiz J, Greenland T, Vandenesch F, Etienne J
机构信息
Centre National de Référence des Staphylocoques, Faculté de Médecine, UPRES EA1655, Lyon, France.
出版信息
J Clin Microbiol. 1998 Apr;36(4):1042-5. doi: 10.1128/JCM.36.4.1042-1045.1998.
Abstract
The presence of staphylococcal superantigenic toxins in the supernatants of liquid cultures was detected by an easy and rapid method assessing the activation of T lymphocytes by cytofluorimetric measurement of CD69 expression. Staphylococcus aureus cells were grown in Eagle's minimum essential medium supplemented with 5% heat-inactivated fetal calf serum. Supernatant fluids from all S. aureus strains producing superantigen-related toxins, including enterotoxins A to E, toxic shock syndrome toxin, and exfoliative toxins A and B, induced CD69 expression in a significantly higher number of T cells than a cutoff of 2%. This CD69 assay might be used for initial detection of superantigens from S. aureus strains isolated in the context of staphylococcal toxemia or related chronic human diseases such as atopic dermatitis or Kawasaki syndrome.
摘要
通过一种简单快速的方法，即通过细胞荧光测定法检测CD69表达来评估T淋巴细胞的活化，从而检测液体培养上清液中葡萄球菌超抗原毒素的存在。金黄色葡萄球菌细胞在补充有5%热灭活胎牛血清的伊格尔最低限度基本培养基中生长。来自所有产生超抗原相关毒素的金黄色葡萄球菌菌株的上清液，包括肠毒素A至E、中毒性休克综合征毒素以及剥脱毒素A和B，诱导CD69表达的T细胞数量显著高于2%的临界值。这种CD69检测方法可用于从葡萄球菌血症或相关慢性人类疾病（如特应性皮炎或川崎综合征）背景下分离出的金黄色葡萄球菌菌株中超抗原的初步检测。
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