Lina G, Fleer A, Etienne J, Greenland T B, Vandenesch F
Départment de Recherche en Bactériologie Médicale (UPR ES), Faculté de Médecine Alexis Carrel, Lyon, France.
FEMS Immunol Med Microbiol. 1996 Jan;13(1):81-6. doi: 10.1111/j.1574-695X.1996.tb00219.x.
Two strains of Staphylococcus epidermidis isolated from patients with toxic shock symptoms have been reported to carry genes related to S. aureus enterotoxins B and C by dot-blot hybridisation, although the corresponding superantigenic toxins were not detected immunologically. We here show that these strains produce no superantigens capable of stimulating proliferation of human mononuclear leukocytes or rabbit splenocytes, and that no DNA homologous to the seb or sec genes can be detected by PCR. However, stimulation of human monocytes by whole killed bacteria induced dose-dependent production of the cytokines TNF alpha, IL-1 beta and IL-6, which may be responsible for the clinical symptoms in these patients.
据报道，从有毒性休克症状患者中分离出的两株表皮葡萄球菌，通过斑点杂交法检测发现其携带与金黄色葡萄球菌肠毒素B和C相关的基因，尽管免疫学检测未发现相应的超抗原毒素。我们在此表明，这些菌株不会产生能够刺激人单核白细胞或兔脾细胞增殖的超抗原，并且通过聚合酶链反应（PCR）无法检测到与seb或sec基因同源的DNA。然而，全菌灭活后刺激人单核细胞会诱导细胞因子TNFα、IL-1β和IL-6呈剂量依赖性产生，这可能是导致这些患者出现临床症状的原因。
