Tanaka T, Umesaki N, Mizuno K, Chang L, Miyama M, Ohtaki S, Ogita S
Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
Horm Metab Res. 1998 Feb;30(2):61-5. doi: 10.1055/s-2007-978835.
Although reports have been published on the generation of transforming growth factor-beta 1 (TGF-beta 1) and expression of TGF-beta receptors in human endometrial tissues, the biological functions of endometrial TGF-beta 1 have remained unclear. In this study, the effects of TGF-beta 1 on endometrial apoptosis were examined by using an endometrial epithelial cell line HHUA which is susceptible to Fas-mediated apoptosis. TGF-beta 1 alone did not affect the cell growth of HHUA, but pretreatment of HHUA with TGF-beta 1 enhanced Fas-mediated growth suppression and DNA fragmentation of the cells. Flow cytometric analyses showed that TGF-beta 1 did not affect Fas expression on the cell surface. These findings lead to the conclusion that TGF-beta 1 enhances susceptibility to Fas-mediated apoptosis in the endometrial epithelial cell. Modulation of Fas-mediated endometrial apoptosis by TGF-beta 1 may thus be a tissue-specific effect which is distinct from other TGF-beta 1 effects on Fas-mediated apoptosis in activated T-cells and rheumatoid synovial cells. The biological functions of endometrial TGF-beta 1 and endometrial apoptosis are also discussed.
尽管已经有关于人子宫内膜组织中转化生长因子-β1（TGF-β1）的产生及TGF-β受体表达的报道，但子宫内膜TGF-β1的生物学功能仍不清楚。在本研究中，利用易受Fas介导凋亡影响的子宫内膜上皮细胞系HHUA，检测了TGF-β1对子宫内膜凋亡的影响。单独的TGF-β1不影响HHUA的细胞生长，但用TGF-β1预处理HHUA可增强Fas介导的细胞生长抑制和DNA片段化。流式细胞术分析表明，TGF-β1不影响细胞表面Fas的表达。这些发现得出结论，TGF-β1增强了子宫内膜上皮细胞对Fas介导凋亡的易感性。因此，TGF-β1对Fas介导的子宫内膜凋亡的调节可能是一种组织特异性效应，不同于TGF-β1对活化T细胞和类风湿滑膜细胞中Fas介导凋亡的其他效应。同时还讨论了子宫内膜TGF-β1的生物学功能及子宫内膜凋亡。
