Monsalve M V, Edin G, Devine D V
Canadian Red Cross Society Blood Services, British Columbia, Vancouver, B.C., Canada.
Hum Immunol. 1998 Jan;59(1):48-55. doi: 10.1016/s0198-8859(97)00251-6.
We analyzed the distribution of HLA class II alleles and haplotypes in one Na-Dene (Athabaskan) group from British Columbia (Canada) by PCR amplification of the DRB1, DQA1 and DQB1 second exon sequences. We extended the typing of the DRB1 in an Amerindian group (Penutian) from British Columbia. The presence of the alleles DRB1* 0405, *0407 and 0410 only in Na-Dene and alleles DRB10408, 13011302, *1304, 1305, 1502 and 1601 only in Amerindians suggests separate origins of these two groups. There were fifteen different DRB1/DQA1/DQB1 haplotypes. One unique haplotype previously reported in Native Americans was found. Thirty-four per cent of Athabaskans presented Native American haplotype DRB11402/DQA10501/DQB10301. In addition, the results of this study are compatible with previous evidence with mitochondrial (mtDNA) polymorphisms indicating that Amerindians and Na-Dene populations derived from different migrations from Asia.
我们通过对DRB1、DQA1和DQB1第二外显子序列进行PCR扩增，分析了来自加拿大不列颠哥伦比亚省的一个纳-德内（阿萨巴斯卡）群体中HLA II类等位基因和单倍型的分布情况。我们对来自不列颠哥伦比亚省的一个美洲印第安群体（佩纽蒂安语族）的DRB1分型进行了扩展。仅在纳-德内群体中存在的等位基因DRB10405、0407和0410，以及仅在美洲印第安群体中存在的等位基因DRB10408、*1301、1302、1304、1305、1502和1601，表明这两个群体有着不同的起源。共有15种不同的DRB1/DQA1/DQB1单倍型。发现了一种先前在美洲原住民中报道过的独特单倍型。34%的阿萨巴斯卡人呈现出美洲原住民单倍型DRB11402/DQA10501/DQB10301。此外，本研究结果与先前关于线粒体（mtDNA）多态性的证据一致，表明美洲印第安人和纳-德内群体源自亚洲不同的移民。
