Bolte G, Wolburg H, Beuermann K, Stocker S, Stern M
University Children's Hospital, Germany.
Clin Chim Acta. 1998 Feb 23;270(2):151-67. doi: 10.1016/s0009-8981(97)00218-0.
A comparison between the intestinal epithelial cell lines Caco-2 and T84 was made to assess the influence of enterocytic differentiation on food protein binding capacities of the brush border membrane. Cell morphology and expression of brush border-associated enzymes were studied as differentiation markers. Food protein binding to isolated brush border membranes was measured with a dot blot chemiluminescence assay. Early at confluence, Caco-2 cells exhibited a more differentiated state compared to T84 cells. Brush border membranes of both cell lines bound gliadin peptides, beta-lactoglobulin and ovalbumin specifically. Binding capacities increased from gliadin peptides to ovalbumin to beta-lactoglobulin. There was correlation of membrane binding capacity with degree of cell differentiation. Due to their similarity to small intestinal epithelial cells, the colon carcinoma cell lines Caco-2 and T84 represent models for studying food protein-enterocytic brush border membrane interactions in relation to varying degrees of cell differentiation.
对肠上皮细胞系Caco-2和T84进行了比较，以评估肠细胞分化对刷状缘膜食物蛋白结合能力的影响。研究了细胞形态和刷状缘相关酶的表达作为分化标志物。用斑点印迹化学发光法测定食物蛋白与分离的刷状缘膜的结合。在汇合早期，与T84细胞相比，Caco-2细胞表现出更分化的状态。两种细胞系的刷状缘膜均特异性结合麦醇溶蛋白肽、β-乳球蛋白和卵清蛋白。结合能力从麦醇溶蛋白肽到卵清蛋白再到β-乳球蛋白逐渐增加。膜结合能力与细胞分化程度相关。由于结肠癌细胞系Caco-2和T84与小肠上皮细胞相似，它们代表了研究食物蛋白与肠细胞刷状缘膜相互作用与不同程度细胞分化关系的模型。
