Freer G, Florio W, Dalla Casa B, Castagna B, Maisetta G, Batoni G, Corsini V, Senesi S, Campa M
Dipartimento di Biomedicina Sperimentale, Infettiva e Pubblica, Università degli Studi di Pisa, Italy.
FEMS Immunol Med Microbiol. 1998 Feb;20(2):129-38. doi: 10.1111/j.1574-695X.1998.tb01119.x.
Effective protection against Mycobacterium tuberculosis may be achieved in experimental animals by immunization with proteins secreted by tuberculous bacilli in the extracellular milieu during growth. In this study, monoclonal antibodies were raised against Mycobacterium bovis bacillus Calmette-Guérin (BCG) culture filtrate proteins or live BCG, in an attempt to identify novel mycobacterial secretion antigens: the localization of the antigens recognized by the monoclonal antibodies within the mycobacterial cell was studied and interspecies reactivity was also investigated. The monoclonal antibodies obtained recognized proteins of molecular mass ranging from 5 to 82 kDa, with a prevailing frequency in the 30 kDa region. Three of the monoclonal antibodies recognized proteins present only in culture filtrates, one reacted with a cytoplasmic antigen, while the remaining antibodies recognized components which were mainly associated with the cell wall and the cytoplasmic membrane. The chemical nature and possible identity of the antigens was checked. Three monoclonal antibodies are likely to react with novel mycobacterial antigens of 5, 42 and 82 kDa, respectively.
通过用结核杆菌生长过程中在细胞外环境中分泌的蛋白质进行免疫，可在实验动物中实现对结核分枝杆菌的有效保护。在本研究中，制备了针对牛分枝杆菌卡介苗（BCG）培养滤液蛋白或活卡介苗的单克隆抗体，以试图鉴定新的分枝杆菌分泌抗原：研究了单克隆抗体识别的抗原在分枝杆菌细胞内的定位，并调查了种间反应性。获得的单克隆抗体识别分子量范围为5至82 kDa的蛋白质，在30 kDa区域频率较高。其中三种单克隆抗体识别仅存在于培养滤液中的蛋白质，一种与细胞质抗原反应，而其余抗体识别主要与细胞壁和细胞质膜相关的成分。检查了抗原的化学性质和可能的身份。三种单克隆抗体可能分别与分子量为5、42和82 kDa的新分枝杆菌抗原反应。
