Drowart A, De Bruyn J, Huygen K, Damiani G, Godfrey H P, Stelandre M, Yernault J C, Van Vooren J P
Université Libre de Bruxelles, Belgium.
Scand J Immunol. 1992 Nov;36(5):697-702. doi: 10.1111/j.1365-3083.1992.tb03130.x.
Nine monoclonal antibodies (MoAbs) were produced against Mycobacterium bovis BCG antigen 85 complex. Using isoelectric focusing combined with Western (immunoblot) blot analysis, antigenically related proteins could be identified in culture filtrates from M. tuberculosis, M. bovis, M. Kansasii, M. avium, M. xenopi, M. gordonae, M. fortuitum, M. phlei and M. smegmatis. Most of the MoAbs were found to be broadly cross-reactive between the various mycobacterial species, albeit some minor differences were observed. These MoAbs reacted generally, in each species, with different components. One MoAb (VID1-14) was found to be specifically directed only against antigen 85B from M. bovis, M. tuberculosis and M. kansasii.
制备了9种针对牛分枝杆菌卡介苗抗原85复合物的单克隆抗体（MoAb）。通过等电聚焦结合蛋白质印迹（免疫印迹）分析，可在结核分枝杆菌、牛分枝杆菌、堪萨斯分枝杆菌、鸟分枝杆菌、蟾蜍分枝杆菌、戈登分枝杆菌、偶然分枝杆菌、草分枝杆菌和耻垢分枝杆菌的培养滤液中鉴定出抗原相关蛋白。发现大多数单克隆抗体在各种分枝杆菌物种之间具有广泛的交叉反应性，尽管观察到了一些细微差异。这些单克隆抗体通常在每个物种中与不同的成分发生反应。发现一种单克隆抗体（VID1-14）仅特异性针对来自牛分枝杆菌、结核分枝杆菌和堪萨斯分枝杆菌的抗原85B。
