Isolation of the O-glycosidically linked oligosaccharides obtained by alkaline borohydride degradation from oviducal mucins of the toad Bufo bufo.

A combination of normal-phase high-performance liquid chromatography (HPLC) on amino-bonded silica and reversed-phase HPLC on octadecylsilica has been used to separate the reduced oligosaccharides produced by alkaline borohydride degradation of oviducal mucins obtained from the jelly coat of Bufo bufo. The former technique provides suitable separation on the basis of molecular size, while the latter method offers selectivity for stereoisomers. Thirty-four compounds, ranging in size from a trisaccharide to a dodecaoligosaccharide, have been isolated preparatively using a Supelcosyl LC-NH2 normal-phase column eluted with aqueous acetonitrile and a Zorbax ODS reversed-phase column eluted with water.