高灵敏度糖蛋白分析中的新型联用方法。
New hyphenated methodologies in high-sensitivity glycoprotein analysis.
作者信息
Novotny Milos V, Mechref Yehia
机构信息
Department of Chemistry, Indiana University, Bloomington 47405, USA.
出版信息
J Sep Sci. 2005 Oct;28(15):1956-68. doi: 10.1002/jssc.200500258.
Abstract
High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.
摘要
高灵敏度糖蛋白分析在现代生物医学和临床研究以及重组蛋白产品的开发中备受关注。本专题综述重点介绍了高灵敏度糖蛋白分析中新联用方法的发展。这些方法尤其包括结合在线全甲基化平台的毛细管液相色谱/基质辅助激光解吸电离/飞行时间/串联飞行时间质谱，以及与质谱联用的基于硅胶的凝集素微柱。这些方法在糖组学和糖蛋白质组学分析中对模型糖蛋白以及生物样品衍生的总糖组和糖蛋白质组的潜力得到了证明。此外，还描述了毛细管电泳-质谱、毛细管电色谱以及带石墨化碳的纳升液相色谱在糖组学和糖蛋白质组学领域的应用。
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