人41 kDa磷酸核糖焦磷酸合成酶相关蛋白的cDNA分子克隆
Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein.
作者信息
Katashima R, Iwahana H, Fujimura M, Yamaoka T, Ishizuka T, Tatibana M, Itakura M
机构信息
Otsuka Department of Clinical and Molecular Nutrition, School of Medicine, University of Tokushima, Japan.
出版信息
Biochim Biophys Acta. 1998 Mar 13;1396(3):245-50. doi: 10.1016/s0167-4781(97)00217-0.
A human cDNA encoding 41-kDa phosphoribosylpyrophosphate (PRPP) synthetase (PRS)-associated protein (PAP41) was cloned from two expressed sequence tag (EST) clones having the nucleotide similarity of 61.5 and 70.0% to human PAP39 cDNA. The predicted open reading frame of 1107 base pairs (bp) has the nucleotide identity of 91.8% to rat PAP41 and encodes a protein of 369 amino acids with a calculated molecular weight (MW) of 40,925. The deduced amino acid sequence exhibits the 98.9% identity to rat PAP41 and 72.2, 50.6, and 50.0% identity with human PAP39, PRS I, and PRS II, respectively, but lacks the PRPP binding site. Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis.
从两个与人类PAP39 cDNA核苷酸相似度分别为61.5%和70.0%的表达序列标签（EST）克隆中，克隆出了一个编码41-kDa磷酸核糖焦磷酸（PRPP）合成酶（PRS）相关蛋白（PAP41）的人类cDNA。预测的1107个碱基对（bp）的开放阅读框与大鼠PAP41的核苷酸同一性为91.8%，编码一个由369个氨基酸组成的蛋白质，计算分子量（MW）为40,925。推导的氨基酸序列与大鼠PAP41的同一性为98.9%，与人类PAP39、PRS I和PRS II的同一性分别为72.2%、50.6%和50.0%，但缺乏PRPP结合位点。Southern印迹分析表明，PAP41基因在人类基因组中以单拷贝形式存在。Northern印迹分析显示，约2.1 kb的单一PAP41 mRNA存在于五种人类细胞系中。
Zotero 插件