Yamagoe S, Kameoka Y, Hashimoto K, Mizuno S, Suzuki K
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo, Japan.
Genomics. 1998 Mar 15;48(3):324-9. doi: 10.1006/geno.1997.5198.
We originally isolated LECT2 (leukocyte cell-derived chemotaxin 2) as a 16-kDa secreted protein having a human neutrophil chemotactic activity, then cloned human and bovine LECT2 cDNAs and demonstrated the liver-specific expression of the protein. LECT2 is thought to be a multifunctional protein, because it was recently found to be identical to chondromodulin-II a growth stimulator of chondrocyte cells. We report here the cloning and the structural analysis of the human LECT2 gene. The gene spans approximately 8 kb and consists of four exons and three introns. Primer extension analysis revealed that several transcription initiation sites occur within 70-230 nucleotides upstream of the translation initiation codon. Several transcriptional control sequences relevant to the liver-specific expression have been identified at the 5' untranslated region of the human LECT2 gene. The human LECT2 gene was mapped to chromosome 5q31.1-q32 by fluorescence in situ hybridization. This region contains a cluster of cytokine genes including IL-4, IL-5, and IL-9.
我们最初将LECT2（白细胞衍生趋化因子2）分离为一种具有人中性粒细胞趋化活性的16 kDa分泌蛋白，随后克隆了人和牛的LECT2 cDNA，并证明了该蛋白的肝脏特异性表达。LECT2被认为是一种多功能蛋白，因为最近发现它与软骨调节素-II相同，软骨调节素-II是软骨细胞的生长刺激因子。我们在此报告人LECT2基因的克隆和结构分析。该基因跨度约8 kb，由四个外显子和三个内含子组成。引物延伸分析表明，在翻译起始密码子上游70-230个核苷酸内有几个转录起始位点。在人LECT2基因的5'非翻译区已鉴定出几个与肝脏特异性表达相关的转录控制序列。通过荧光原位杂交将人LECT2基因定位到染色体5q31.1-q32。该区域包含一组细胞因子基因，包括IL-4、IL-5和IL-9。
