大鼠和人类中B29/Ig-β（CD79B）基因与骨骼肌、钠通道及生长激素基因的物理连锁。

Physical linkage of the B29/Ig-beta (CD79B) gene to the skeletal muscle, sodium-channel, and growth hormone genes in rat and human.

作者信息

Nakazato S, Nomoto K, Kazahari K, Ono M

机构信息

Department of Molecular Biology, School of Medicine Kitasato University, Kanagawa, Japan.

出版信息

Genomics. 1998 Mar 15;48(3):363-8. doi: 10.1006/geno.1997.5178.

DOI:10.1006/geno.1997.5178

PMID:9545642

Abstract

In the region between the polyadenylation site of the rat skeletal muscle (SkM) Na-channel gene and the 5' end of the growth hormone (GH) gene, a gene coding for B-cell-specific membrane protein B29/Ig-beta was found and noted to have the same orientation as the Na-channel and GH genes. Rat B29/Ig-beta gene was 3.1 kb in length with six exons and was separated by 3.3 and 9.3 kb from Na-channel and GH genes, respectively. Rat B29/Ig-beta protein comprised 228 amino acids, and its amino acid sequence was 85 and 69% identical with the mouse and human counterparts, respectively. With the long-area PCR method, genomic DNA connecting human SkM Na-channel (SCN4A) and B29/Ig-beta (CD79B) genes and CD79B and GH (GH1) genes was amplified, and the physical linkage of SCN4A/CD79B/ GH1 genes in the human genome was established. The human CD79B gene was separated by 6.3 and 10.5 kb from the SCN4A and GH1 genes, respectively.

摘要

在大鼠骨骼肌（SkM）钠通道基因的聚腺苷酸化位点与生长激素（GH）基因的5'端之间的区域，发现了一个编码B细胞特异性膜蛋白B29/Ig-β的基因，并且该基因与钠通道和GH基因具有相同的方向。大鼠B29/Ig-β基因长度为3.1 kb，有六个外显子，分别与钠通道基因和GH基因间隔3.3 kb和9.3 kb。大鼠B29/Ig-β蛋白由228个氨基酸组成，其氨基酸序列与小鼠和人类相应蛋白的同源性分别为85%和69%。采用长片段PCR方法，扩增了连接人类SkM钠通道（SCN4A）和B29/Ig-β（CD79B）基因以及CD79B和GH（GH1）基因的基因组DNA，从而确定了人类基因组中SCN4A/CD79B/GH1基因的物理连锁关系。人类CD79B基因分别与SCN4A基因和GH1基因间隔6.3 kb和10.5 kb。