Green B N, Kuchumov A R, Hankeln T, Schmidt E R, Bergtrom G, Vinogradov S N
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Biochim Biophys Acta. 1998 Mar 3;1383(1):143-50. doi: 10.1016/s0167-4838(97)00195-7.
The aquatic larvae of the dipteran, Chironomus thummi thummi contain extracellular hemoglobins which exhibit stage-specific expression. We have used maximum entropy-based deconvolution of the complex, multiply charged electrospray ionization mass spectra, to demonstrate the presence of more than 20 components, ranging in mass from 14,417.3 Da to 17,356.5 Da in the 4th instar larvae. Of the 15 major peaks with intensities > 10 relative to 100 for the 14,417.3 Da-component (CTT-IV), only the 15,528.2-Da peak does not correspond to a known amino acid sequence. Since the number of C. thummi thummi globin genes now stands at 27, including one cDNA and not counting three that must encode known globins, our results suggest that only a limited number of the globin genes are expressed in the 4th instar larvae.
双翅目昆虫摇蚊（Chironomus thummi thummi）的水生幼虫含有细胞外血红蛋白，这些血红蛋白表现出阶段特异性表达。我们利用基于最大熵的复杂多电荷电喷雾电离质谱反褶积技术，证明了四龄幼虫中存在20多种成分，质量范围从14417.3道尔顿到17356.5道尔顿。相对于14417.3道尔顿成分（CTT-IV）的100而言，强度>10的15个主要峰中，只有15528.2道尔顿的峰与已知氨基酸序列不对应。由于目前摇蚊血红蛋白基因的数量为27个，包括一个cDNA，且不计算三个必定编码已知球蛋白的基因，我们的结果表明，在四龄幼虫中只有有限数量的球蛋白基因表达。
