一种改良的用于幽门螺杆菌的麦克马伦染色法：一种高对比度、视觉上突出的方法。
A modified McMullen's staining for Helicobacter pylori: a high-contrast, visibly prominent method.
作者信息
Iwaki H, Sugiyama T, Asaka M
机构信息
Department of Clinical Pathology, Sapporo Medical University, School of Medicine, Hokkaido University, Japan.
出版信息
Helicobacter. 1998 Mar;3(1):45-8. doi: 10.1046/j.1523-5378.1998.08013.x.
BACKGROUND
Many stains can be used for the histological assessment of Helicobacter pylori, but all have defects from the standpoint of sensitivity, convenience, stability, visible prominence and cost. This paper describes a stain which produces satisfactory results on all the above accounts.
MATERIALS AND METHODS
Paraffin-embedded sections were dewaxed and treated with 0.2% w/v carbol fuchsin for two min; then 1.0% w/v malachite green was applied for two minutes.
RESULTS
This technique stained the bacterial from a deep magenta against a light green background. The characteristic morphology of H. pylori was identified very easily as a stumpy curved rod, and the coccoid forms were also identified. Staining was not specific with mixed samples of bacteria, but it was sufficient to identify H. pylori in the gastric mucosa.
CONCLUSION
We have established an easy, stable, convenient, low-cost, and easily recognizable staining method for H. pylori in paraffin-embedded tissue.
背景
许多染色方法可用于幽门螺杆菌的组织学评估，但从敏感性、便利性、稳定性、显色清晰度和成本方面来看，所有方法都存在缺陷。本文介绍了一种在上述所有方面均能产生满意结果的染色方法。
材料与方法
将石蜡包埋切片脱蜡，用0.2%（重量/体积）碱性品红处理两分钟；然后用1.0%（重量/体积）孔雀绿处理两分钟。
结果
该技术将细菌染成深紫红色，背景为浅绿色。幽门螺杆菌的特征形态很容易被识别为短粗弯曲杆菌，并且也能识别出球菌形态。对混合细菌样本染色不具有特异性，但足以在胃黏膜中识别幽门螺杆菌。
结论
我们已经建立了一种用于石蜡包埋组织中幽门螺杆菌的简便、稳定、便捷、低成本且易于识别的染色方法。
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